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目的:探讨利用斑点金免疫渗滤试验﹙DIFGA﹚检测结核分枝杆菌抗体对肺结核临床诊断的应用价值。方法:应用斑点金免疫渗滤法检测了正常人和各类肺部疾病患者共313例血清中结核分枝杆菌抗体,并与痰液涂片法、结核菌培养法和PCR法进行方法学比较。结果:结核分枝杆菌抗体检测肺结核患者阳性率为80.77%,非结核性肺病患者阳性率为7.64%,正常人阳性率为3.08%。结论:斑点金免疫渗滤法检测结核分枝杆菌抗体对肺结核的诊断敏感性高,特异性好,对肺结核诊断有较高的应用价值,并且操作简便、快速,与涂片法、培养法和PCR相比,有明显优势。
Objective: To investigate the value of detecting Mycobacterium tuberculosis antibody in clinical diagnosis of pulmonary tuberculosis by Dot-immunofluorescence assay (DIFGA). Methods: Dot blotting assay was used to detect Mycobacterium tuberculosis antibodies in 313 serum samples of normal subjects and various types of pulmonary disease patients. The method was compared with sputum smear method, Mycobacterium tuberculosis culture method and PCR method . Results: The positive rate of Mycobacterium tuberculosis in detecting tuberculosis was 80.77%, the positive rate in non-tuberculosis patients was 7.64%, and the positive rate was 3.08%. Conclusions: The detection of Mycobacterium tuberculosis antibody by Dot-immunogold filtration assay is highly sensitive and specific for the diagnosis of pulmonary tuberculosis. It has high application value in the diagnosis of pulmonary tuberculosis, and is simple and rapid in operation. Compared with smear method, culture method and Compared to PCR, there is a clear advantage.