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目的 观察补锌对急性冷应激大鼠 ACTH分泌及小鼠耐寒力的影响 .方法 雄性 SD大鼠 32只 ,随机分为 4组 ,1,3组给予正常饲料 ,饮自来水 ,2 ,4组给予正常饲料 ,饮高锌自来水 (补锌 1mg· kg- 1 · d- 1 ) ,5 d后 ,3,4组于 - 15℃冷暴 6 0 min后与 1,2组一同宰杀 ,测血浆 ACTH,血清、肝脏和骨骼肌锌含量 .雄性昆明种小白鼠 40只 ,随机分为 2组 ,1组正常进水进食 ,2组饮高锌水 (补锌 1mg· kg- 1·d- 1 ) ,5 d后 ,两组小鼠同时暴露于 - 15℃的冷室中 ,记录死亡时间 .结果 补锌可以使正常大鼠血浆 ACTH水平升高 (P<0 .0 5 ) ,- 15℃冷暴 6 0 min可使大鼠 ACTH水平明显升高(P<0 .0 1) ,补锌可以降低其升高的幅度 .补锌使正常大鼠肝和骨骼肌锌含量升高 ,并减轻冷暴大鼠肝和骨骼肌的锌含量降低的程度 .补锌组小鼠在 - 15℃冷室内的存活时间比未补锌组延长 15 .9% (P<0 .0 5 ) .结论 适量补锌可能提高机体的耐寒能力
Objective To observe the effect of zinc supplementation on ACTH secretion and cold tolerance in acute cold stress rats.Methods 32 male SD rats were randomly divided into 4 groups, 1, 3 groups were given normal diet, drinking tap water, 2, 4 groups After 5 days, groups 3 and 4 were slaughtered together with groups 1 and 2 at 60 min after cold shock at -15 ° C, and plasma was assayed for plasma zinc (1 mg · kg-1 · d-1) ACTH, serum, liver and skeletal muscle.A total of 40 male Kunming mice were randomly divided into 2 groups, 1 group fed with normal water intake, 2 groups received high zinc water (zinc supplementation 1 mg · kg -1 · d -1 ), After 5 days, both groups of mice were simultaneously exposed to cold room at -15 ℃, and the time of death was recorded.Results Zinc supplementation could increase plasma ACTH level in normal rats (P <0.05), -15 ℃ After 6 min of cold exposure, the level of ACTH in rats increased significantly (P <0.01), and zinc supplementation decreased the increase of ACTH level.Zinc supplementation increased and decreased the zinc content of liver and skeletal muscle in normal rats The level of zinc in the liver and skeletal muscle of cold-stunted rats was decreased.The survival time of zinc-supplemented mice in the cold room at -15 ℃ was 15.9% longer than that in the non-supplemented group (P <0.05) Zinc supplementation may improve the body Tolerance to cold