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目的通过体外实验,观察利注射液炎琥宁注射液对肠道病毒C型病毒在横纹肌肉瘤传代细胞(RD-A细胞)复制的抑制作用和体外灭活作用。方法使用从病人咽拭子样本中分离的病毒,通过三种体外细胞实验:药物在细胞中抑制病毒CVA16复制的药效测定实验;药物对细胞的保护作用实验;药物体外对CVA16病毒的灭活作用的药效测定实验。观察炎琥宁注射液对CVA16病毒感染横纹肌肉瘤传代细胞(RD.A细胞)中所起的作用。结果在炎琥宁注射液在细胞中抑制CVA16病毒复制的药效测定实验中1,:640炎琥宁注射液稀释度组较病毒对照组延迟病变出现2 d,细胞生长未受到影响。在药物对细胞的保护作用实验中l,:8炎琥宁稀释度组较病毒对照组延迟病变出现4 d。在炎琥宁注射液药物体外对CVA16病毒的灭活作用的药效测定研究中1,:40稀释度组较病毒对照组延迟病变出现2 d,而细胞生长未受到影响。结论炎琥宁注射液在体外有抑制CVA16病毒复制和部分灭活病毒的作用;并有一定的保护细胞抑制CVA16病毒感染的作用。本研究用于临床上CVA16感染的早期预防及治疗具有一定的指导意义。
OBJECTIVE: To observe the inhibitory effect and in vitro inactivation of Yihuning injection on replication of rhabdomyosarcoma cell line RD-A cells by in vitro experiments. Methods The virus isolated from a patient’s throat swab sample was used to pass three in vitro cell assays: a pharmacodynamic assay to inhibit the replication of the virus CVA16 in cells; a drug-to-cell protective experiment; the in vitro inactivation of the CVA16 virus by the drug Effect of the efficacy test. To observe the effect of Yanhuoning injection on CVA16 virus infected rhabdomyosarcoma passage cells (RD.A cells). Results Yan Yanning injection in the cell inhibition of CVA16 virus replication in the efficacy of the assay 1: 640 Yanhuning injection dilution group compared with the virus control group delayed 2d, cell growth was not affected. In the protective effect of drugs on the cell l: 8 Yanhuning dilution group compared with the virus control group delayed 4d. In the pharmacodynamic test of inactivation of CVA16 virus by inhbyin injection drug in vitro, delayed cell proliferation was not observed in the 1: 40 dilution group compared with the virus control group for 2 days. Conclusion Yanhuning injection can inhibit the replication of CVA16 virus and partially inactivate the virus in vitro, and protect the cells against CVA16 virus infection in some extent. This study is of clinical significance for the early prevention and treatment of CVA16 infection.