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妊娠７～１１ｄ的孕鼠，经腹腔连续注入氯化甲基汞（２ｍｇ／ｋｇ体重），采用Ｈｅｗｉｓｈ和Ｂｕｒｇｏｙｎｅ法分离纯化不同发育阶段新生大鼠的脑细胞核，来探讨氯化甲基汞对其脑染色质结构的影响。接触或不接触氯化甲基汞的大鼠脑细胞核分别经ＤＮａｓｅ１和ＥｃｏＲ１核酸内切酶消化后进行缺口翻译。结果接触氯化甲基汞生后３ｄ的大鼠脑细胞核３Ｈ－ｄＡＭＰ的掺入量明显低于相应未接触氯化甲基汞的对照组（Ｐ＜０．０５）。我们认为氯化甲基汞对大鼠发育过程中脑染色质结构的影响可能与胎、幼鼠对甲基汞神经毒性的易感有密切关系。
Gestational 7 ~ 11d pregnancy pregnant rats, continuous injection of methylmercury chloride (2mg / kg body weight), Hewish and Burgoyne method was used to separate and purify brain cells in different developmental stages of neonatal rat brain to explore methylmercury chloride Effects of brain chromatin structure. Rat brain nuclei exposed or not exposed to methylmercuric chloride were gapped translated after DNase1 and EcoR1 endonuclease digestion, respectively. Results 3H-dAMP incorporation in rat brain cells exposed to methylmercury chloride was significantly lower than that of the control group exposed to methylmercury chloride (P <0.05). In our opinion, the effect of methylmercury chloride on the chromatin structure during the development of rats may be closely related to the neurotoxicity of methylmercury in young rats and fetuses.