论文部分内容阅读
目的:探讨化岩胶囊药物血清对人骨肉瘤细胞系MG-63诱导的鸡胚绒毛尿囊膜血管的生成、肿瘤的生长及CAM组织中血管内皮生长因子(VEGF)、环氧化酶2(COX-2)表达的影响。方法:以SD大鼠药物喂养7天,制备药物血清及空白血清。孵化9天的种蛋30个以鸡胚绒毛尿囊膜内接种MG-63系骨肉瘤细胞悬液100ul(浓度为18.6×106)的方法造模,分为化岩胶囊药物血清组(HX)、造模空白血清(KX)、造模培养液组(ZM)三组,另取10个开窗非造模形成空白对照组(KB),通过明胶海绵载体每天给予药物血清、空白血清及培养液干预。每天记录CAM的血管数目、形态、肿瘤生长情况等,并拍照图像分析血管直径和面积。连续观察3天后取CAM标本、肿瘤组织给予切片染色及VEGF、COX-2表达检测。结果:造模与非造模组血管面积差异有统计学意义(P<0.05),说明骨肉瘤MG-63细胞系细胞能促进CAM中大中血管的生成,属1级血管者多,药物血清对CAM血管作用具有抑制作用趋势;药物血清组可抑制血管生成(与ZM组比较P<0.05),且能减少血管异生现象;肿瘤组织及CAM中VEGF、COX-2阳性表达率较空白KB组有差异(P<0.05),但各组间无明显差异(P>0.05)。结论:化岩胶囊药物血清具有抑制骨肉瘤细胞诱导的CAM血管生成作用,但似不是通过影响VEGF和COX-2表达机制发生作用,且中药复方抑制血管生成作用可能更为复杂。
OBJECTIVE: To investigate the formation and growth of chicken chorioallantoic membrane induced by MG-63 in human osteosarcoma cell line and the vascular endothelial growth factor (VEGF) and cyclooxygenase 2 (COX) in CAM tissue. -2) The effect of expression. Methods: The rats were fed with SD rats for 7 days to prepare drug serum and blank serum. Thirty eggs hatched for 9 days were immersed in chicken embryo chorioallantoic membranes to inoculate 100 μg of MG-63 osteosarcoma cell suspension (with a concentration of 18.6×106), and were divided into Huayan capsule drug serum group (HX). Three groups of blank serum (KX) and model culture medium (ZM) were created. Ten open windows were used to create a blank control group (KB). Drug serum, blank serum, and culture medium were given daily via a gelatin sponge carrier. Intervention. The number, morphology, and tumor growth of the CAM were recorded every day, and photographs were taken to analyze the diameter and area of the blood vessels. After 3 days of continuous observation, CAM specimens, tumor tissue sections, and VEGF and COX-2 expression were detected. RESULTS: There was a statistically significant difference in vascular area between the model group and the non-model group (P<0.05), indicating that the osteosarcoma MG-63 cell line cells can promote the generation of large and medium blood vessels in CAM, and there are more than 1 level vascular and drug serum. The inhibitory effect on CAM vascular effect was observed. The sera of drug group inhibited angiogenesis (P<0.05 compared with ZM group) and reduced vascular xenogeneosis. The positive expression rate of VEGF and COX-2 in tumor tissue and CAM was higher than that of blank KB. There was a difference in the groups (P<0.05), but there was no significant difference among the groups (P>0.05). Conclusion: The drug serum of Huayan capsule can inhibit the angiogenesis of CAM induced by osteosarcoma cells, but it does not appear to affect the expression mechanism of VEGF and COX-2, and the inhibitory effect of traditional Chinese medicine compound on angiogenesis may be more complicated.