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目的 :探讨白介素 12 (IL - 12 )诱导T细胞凋亡过程中对T细胞的Fas/FasL和TNFR/TNFα表达的影响作用。方法 :dUTP缺口末端标记法和AnnexinV法 ;用FITC标记TNFR1,PE标记CD95 ,生物素标记FasL ,流式细胞仪检测 3种T细胞 (人淋巴瘤T细胞株HTB176、人急性白血病T细胞株TIB15 2和正常人T细胞 )的百分率 ;用半定量PCR的方法检测FasL和TNFα的mRNA的表达作用。结果 :HTB176和TIB15 2的细胞在IL - 12处理 1h ,生物素标记FasL百分率和FasL的mRNA表达率开始增加 ,2 4h达到高峰 ,但正常T细胞在 2 4h后才有反应 ;正常T细胞经IL -12处理 1h内细胞FasL百分率和FasL的mRNA表达率开始增加 ,在以后的试验期 6、12、2 4h始终保持恒定水平 ;但 3种T细胞在IL - 12作用下不促进细胞CD95和TNFR1及TNFα的表达。结论 :IL - 12诱导T细胞凋亡中有许多调节因子的协调作用 ,但作用机制是不同的
AIM: To investigate the effect of interleukin - 12 (IL - 12) on T cell apoptosis induced by Fas / FasL and TNFR / TNFα. Methods: The dUTP nick end labeling method and AnnexinV method were used. Tumor necrosis factor-α (TNFR1), PE-labeled CD95 and PE-labeled FasL were detected by FITC and flow cytometry (T lymphocyte cell line HTB176, TIB15 2 and normal human T cells). The mRNA expression of FasL and TNFα was detected by semi-quantitative PCR. Results: After treatment with IL - 12 for 1 h, the percentage of biotin - labeled FasL and the expression of FasL mRNA in HTB176 and TIB15 2 cells began to increase, reaching the peak at 24 h. However, normal T cells reacted only after 24 h. Normal T cells The percentage of FasL and the expression of FasL mRNA increased within 1 h after treatment with IL-12, and remained constant at 6, 12 and 24 hours after the treatment with IL-12. However, the effect of IL-12 on the expression of CD95 and FasL TNFR1 and TNFα expression. Conclusion: IL - 12 induces T cell apoptosis in a number of regulatory factors but its mechanism of action is different