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目的:建立用HPLC测定小花黄堇中原阿片碱含量的方法。方法:Gemini C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-三乙胺醋酸溶液(20∶80);流速1.0 mL.min-1;检测波长289 nm;柱温30℃。结果:小花黄堇中原阿片碱的平均质量分数0.905%,原阿片碱在0.124~1.36μg线性关系良好,平均回收率98.49%,RSD 1.9%(n=6)。结论:该方法操作简便,重复性较好,测定结果较为可靠,可用于测定小花黄堇中原阿片碱的含量。
Objective: To establish a HPLC method for the determination of protopine in small flower yellow pansy. METHODS: Gemini C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was acetonitrile-triethylamine acetate solution (20:80). The flow rate was 1.0 mL.min-1. The detection wavelength was 289 nm. Results: The average mass fraction of protopine in Huang Hua was 0.905%. The linearity of protopine in 0.124 ~ 1.36μg was good with the average recovery of 98.49% and RSD of 1.9% (n = 6). Conclusion: The method is simple, reproducible and reproducible. The assay is reliable and can be used to determine the content of protopine in Pistacia chinensis.