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目的探讨膀胱尿路上皮癌中PTEN基因启动子甲基化与PI3K/Akt信号传导通路的关系。方法应用甲基化特异性PCR MSP、Western Blot方法,检测41例膀胱尿路上皮癌和18例正常膀胱组织中PTEN基因启动子甲基化状态,并进一步分析其与PI3K/Akt信号转导通路的相关性。结果膀胱尿路上皮癌中PTEN基因启动子甲基化率为53.66%(22/41),明显高于正常膀胱组织的0%(0/18),2组间差异有统计学意义(P<0.01);在41例膀胱尿路上皮癌组织中,PTEN甲基化阳性组P-Akt的相对表达量为(1.86±0.13),显著高于阴性组(0.92±0.11),二者差异有统计学意义(P<0.05),而2组间Akt的相对表达量则无明显差异。结论膀胱尿路上皮癌中PTEN基因启动子存在高甲基化状态,且与PI3K/Akt信号转导通路蛋白表达呈正相关。
Objective To investigate the relationship between PTEN promoter methylation and PI3K / Akt signaling pathway in bladder urothelial carcinoma. Methods Methylation-specific PCR MSP and Western Blot were used to detect the promoter methylation status of PTEN gene in 41 cases of urothelial carcinoma of bladder and 18 cases of normal bladder tissue, and further analyzed the relationship between PI3K / Akt signal transduction pathway Relevance. Results The promoter methylation rate of PTEN gene in bladder urothelial carcinoma was 53.66% (22/41), which was significantly higher than that in normal bladder tissue (0%, 0/18), the difference was statistically significant (P < 0.01). The relative expression of P-Akt in PTEN methylation-positive group was (1.86 ± 0.13) in 41 cases of bladder urothelial carcinoma, which was significantly higher than that in the negative group (0.92 ± 0.11) (P <0.05), while the relative expression of Akt between two groups had no significant difference. Conclusions PTEN gene promoter is hypermethylated in bladder urothelial carcinoma and positively correlated with PI3K / Akt signal transduction pathway.