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目的：探讨ｐ１６抑癌基因与卵巢浆液性癌发生、发展及其生物学特征的关系。方法：应用非放射性原位杂交技术对４７例卵巢上皮性浆液性肿瘤组织（良性１０例、交界性４例、癌３３例）和８例正常卵巢组织中ｐ１６ｍＲ－ＮＡ进行检测。结果：ｐ１６ｍＲＮＡ表达主要位于胞浆内呈红色颗粒状，阳性表达细胞多呈灶状分布。ｐ１６ｍＲＮＡ在卵巢浆液性癌中检出率（４８．５％），低于良性浆液性瘤（９０％）及正常卵巢组织（１００％）（Ｐ均＜０．０５）。结论：ｐ１６ｍＲ－ＮＡ低表达与卵巢浆液性癌的恶性程度高、肿瘤分化差、癌瘤播散及淋巴结转移有关（Ｐ＜０．０５或Ｐ＜０．０１）。ｐ１６作为一种新型抑癌基因，其ｍＲＮＡ低表达可能与卵巢浆液性癌发生、发展密切相关。
Objective: To investigate the relationship between p16 tumor suppressor gene and the occurrence, development and biological characteristics of ovarian serous carcinoma. Methods: The non-radioactive in situ hybridization was used to detect p16mR-NA in 47 cases of ovarian epithelial serous tumor tissues (benign 10 cases, borderline 4 cases, cancer 33 cases) and 8 cases of normal ovarian tissues. Results: The expression of p16 mRNA was mainly located in the cytoplasm with red granule, and the positive cells showed mostly foci. The positive rate of p16 mRNA in ovarian serous carcinoma (48.5%) was lower than that in benign serous tumors (90%) and normal ovarian tissues (100%) (all P <0.05). Conclusion: The low expression of p16mR-NA is associated with the high malignant degree of ovarian serous carcinoma, poor differentiation of tumor, dissemination of cancer and lymph node metastasis (P <0.05 or P <0.01). As a novel tumor suppressor gene, the low expression of p16 may be closely related to the occurrence and development of ovarian serous carcinoma.