The Annonaccac family is a main one of the tropical plants flora and there are totally 127 genus, about 2231 species in the world, wildly distributed in the tropical zone and subtropics areas.China is one of the countries where species of this family are mostly distributed, with 24 genus, 103 species and 6 variant, among which, 45 species and 1 variant are endemic to China. There are about 100 types of plants of Artabotrys genus in the world, among which, 10 species are in China. Scholars from home and abroad have found a series of new substance of biological activities in the plants of Annonaccac family with antimalarial, anti-tumor, anti-leukemia and obvious cardiotonic effects,which has aroused great interest. Plants of Artabotrys genus, Artabotrys Hainanensis are endemic to Hainan, distributed widely in Hainan Island. They have long been used among the ordinary people as medicinal plants with antipyretic, antidotal, antiphlogistic and analgesic effects. The flavonoid is a very important secondary metabolite in plants with many physiological activities such as fat-reduction, antithrombus, antioxidant and blood-sugar-reduction. This paper studies the total flavonoids from stem and leaf of Artabotrys by spectrophotometry. Artabotrys Hainanensis R.E.Fries were collected from Hainan Jianfeng Mountain and were identified. Spectrophotometry was used to determination of the total flavonoids from stem and leaf of Artabotrys. The total flavonoids of Artabotrys were tested with rutin as check sample. By adding aluminium ion agent and controling proper pH, the flavonoids and the aluminium ion were combined into complex compound and the characteristic absorption peak was obtainable in the visible spectrum. The total flavonoids from stem and leaf of Artabotrys were determined to be 2.29% in stem and 6.83% in leaf. The regression equation was Y=0.000786A+0.0058, and the correlation coefficient r=0.9997. This method is easily operated and the result determined is stable and accurate.