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目的 探讨神经细胞粘附分子 (NCAM)对恶性胶质瘤细胞侵袭行为的影响。方法 用脂质体法将NCAMcDNA转染人胶质瘤细胞株U2 5 1MG ,体外波艾登 (Boyden)小室中培养 7h ,同时用抗NCAM单克隆抗体 (1∶30稀释 )封闭细胞表面的NCAM作对照 ,观察转染前后U2 5 1MG细胞及U87MG细胞侵袭基质凝胶能力的变化规律。结果 转染后U2 5 1MG细胞表达NCAM ,侵袭能力(10 3 .0 0± 37.0 0 )较转染前 (2 2 3 .6 7± 40 .2 8)减弱 (P <0 .0 5 ) ,对照组U87MG细胞体外侵袭能力(70 .0 0± 2 0 .95 )亦比转染前U2 5 1MG细胞低 (P <0 .0 1) ,经抗NCAM单克隆抗体封闭细胞表面NCAM抗原后 ,U87MG细胞及转染后U2 5 1MG细胞侵袭力均比抗体处理前增强 (P <0 .0 5 )。结论 NCAM对恶性胶质瘤细胞体外侵袭能力起抑制作用
Objective To investigate the effect of neural cell adhesion molecule (NCAM) on the invasive behavior of malignant glioma cells. METHODS: The NCAM cDNA was transfected into human glioma cell line U2 5 1MG by liposome method and cultured in Boyden chamber for 7 h in vitro. At the same time, NCAM was blocked on the cell surface with anti-NCAM monoclonal antibody (diluted 1:30). As a control, the changes of gelation ability of U2 5 1MG cells and U87MG cells invading the matrix before and after transfection were observed. Results After transfection, UCAM cells expressed NCAM, and the invasive ability (10 3 0 ±37.0 0) was weaker than that before transfection (2 2 3.67 ± 40.28) (P <0.05). In vitro invasion of U87MG cells in the control group (70.0%±20.95%) was also lower than that of U2511-5MG cells before transfection (P < 0.01). After blocking the NCAM antigen on the cell surface by the anti-NCAM monoclonal antibody, The invasiveness of U251MG cells after U87MG cells and transfection was stronger than that before antibody treatment (P < 0.05). Conclusion NCAM inhibits the invasion of malignant glioma cells in vitro