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目的:研究原儿茶醛缩氨基硫脲铜(II)配合物(PTC)对高脂血症模型大鼠血脂、主动脉组织形态学及肝组织抗氧化活性的影响.方法:60只SD大鼠随机分为6组(每组10只),即正常对照组、高脂血症模型组、药物对照组(即费非诺贝特组,0.04g/kg),PTC小剂量组(0.005g/kg),PTC中剂量组(0.05g/kg),PTC大剂量组(0.25g/kg).在以高脂饲料喂养5wk开始,正常对照组和高脂血症模型组给以5g/L羧甲基纤维素钠水溶液10mL/kg体质量灌胃.其他实验组分别给以5g/L羧甲基纤维素钠水溶液配成药物混悬液灌胃,每日一次.实验到10wk后全自动生化分析仪测定各组动物血脂,HE染色做病理切片观察大鼠主动脉形态学变化,同时测定肝组织超氧化物歧化酶(SOD),过氧化氢酶(CAT)活性及抑制羟自由基(.OH)的能力.结果:原儿茶醛缩氨基硫脲铜(II)配合物能使模型大鼠肝组织清除羟自由基(.OH)的能力提高(P<0.01),SOD的活性增强(P<0.01或0.05);各剂量组均有降低血清胆固醇和提高血清高密度脂蛋白(HDL-C)含量的趋势,但无统计学意义.结论:原儿茶醛缩氨基硫脲铜(II)配合物在实验动物体内的确有清除超氧阴离子自由基(O2.-)和羟自由基(.OH)的双功能作用.
Objective: To study the effect of protocatechuic thiosemicarbazone copper (II) complex (PTC) on the blood lipid, aorta histomorphology and liver tissue anti-oxidative activity in hyperlipidemic rats.Methods: Sixty SD rats The rats were randomly divided into 6 groups (10 in each group), namely normal control group, hyperlipidemia model group, drug control group (ie, fenofibrate group, 0.04g / kg), PTC low dose group / kg), PTC medium dose group (0.05g / kg) and PTC high dose group (0.25g / kg) .After feeding with high fat diet for 5 weeks, the normal control group and hyperlipidemia model group were given 5g / Sodium carboxymethyl cellulose solution 10mL / kg body weight gavage.Other experimental groups were given 5g / L aqueous solution of sodium carboxymethyl cellulose dubbed drug suspension gavage once a day.Experiments to 10wk after the automatic The biochemical analyzer was used to determine the blood lipids in each group. HE staining was used to observe the morphological changes of the aorta in rats. The activities of superoxide dismutase (SOD), catalase (CAT), and hydroxyl radical .OH) .Results: The protocatechuic aldehyde thiosemicarbazone copper complex can improve the ability of scavenging hydroxyl radical (.OH) (P <0.01) and increase the activity of SOD (P <0. 01 or 0.05) .There was a trend of decreasing serum cholesterol and HDL-C in each dose group, but there was no statistical significance.Conclusion: The protocatechuic thiosemicarbazone copper In experimental animals, there is indeed a bifunctional effect of scavenging superoxide anion radicals (O2.-) and hydroxyl radicals (.OH).