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目的研究人乳头状瘤病毒 HPV-E6和 p53在食管鳞癌中的表达,以探讨它们在食管鳞癌中发生发展的生物学意义.方法应用 LSAB 免疫组化方法对16例正常食管粘膜,18例慢性炎症,22例不典型增生以及60例食管鳞癌组织进行HPV-E6和 P53蛋白表达的研究.结果在正常食管粘膜、慢性炎症、不典型增生及食管鳞癌中HPV-E6蛋白阳性率分别为37%,39%,50%及73%,P53蛋白阳性率在上述四组中则为0%,6%,41%及63%.四组比较,HPV-E6和 P53蛋白表达均有显著差别(P<0.05).在60例食管鳞癌Ⅰ,Ⅱ,Ⅲ级分组中,HPV-E6阳性率分别为59%,78%和85%,p53阳性率则分别为45%,67%,80%.三个分级组比较,HPV-E6和 P53蛋白表达均有显著差别(P<0.05).结论 HPV-E6及 p53与食管鳞癌的发生发展有着一定相关性,且 HPV-E6及 p53共同作用很可能更为食管鳞癌发生发展的重要因素.
Objective To investigate the expression of human papillomavirus HPV-E6 and p53 in esophageal squamous cell carcinoma and to explore their biological significance in the development of esophageal squamous cell carcinoma. Methods LSAB immunohistochemistry was used on 16 normal esophageal mucosa, 18 Cases of chronic inflammation, 22 cases of atypical hyperplasia and 60 cases of esophageal squamous cell carcinoma were studied for the expression of HPV-E6 and P53 protein. Results The positive rate of HPV-E6 protein in normal esophageal mucosa, chronic inflammation, atypical hyperplasia, and esophageal squamous cell carcinoma The positive rate of P53 protein in the above four groups was 0%, 6%, 41%, and 63%, respectively. The expression of HPV-E6 and P53 protein was higher in the four groups. Significant difference (P<0.05). In 60 cases of esophageal squamous cell carcinoma I, II, and III groups, the positive rates of HPV-E6 were 59%, 78%, and 85%, respectively, and the p53 positive rates were 45% and 67%, respectively. 80%. The expression of HPV-E6 and P53 were significantly different in the three graded groups (P<0.05). Conclusion HPV-E6 and p53 have a certain correlation with the occurrence and development of esophageal squamous cell carcinoma, and HPV-E6 and The joint action of p53 is likely to be more important factors in the development of esophageal squamous cell carcinoma.