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检测4种不同产地的山药——河南焦作的“铁棍山药”、山东菏泽的“陈集山药”、湖北武穴的“佛手山药”和江西瑞昌的“南阳山药”中尿囊素的含量。采用超声辅助提取,反相高效液相色谱法进行检测,色谱柱为PhenomenexLunaC18(250mm×4.6mm,5μm);流动相为甲醇-水(1∶9,V/V);流速0.5mL/min;柱温为室温;检测波长224nm。结果表明:尿囊素在1.596～7.182μg线性关系良好,R_2=0.9995;尿囊素的平均回收率为97.2%,RSD=1.20%(n=3)。该方法稳定性、精密度和加样回收率都良好,可以为山药质量控制提供可靠手段,各产地山药中尿囊素含量差异较大,其中南阳山药的尿囊素含量最高,比含量最低的铁棍山药高出124%。
Detection of yam in four different producing areas - “Iron Mountain Yam” in Jiaozuo of Henan Province, “Chen Yam” in Heze of Shandong Province, “Bergam Yam” in Wuxue of Hubei Province and “Nanyang Yam of Jiangxi Ruichang” "Allantoin content. The samples were separated on a Phenomenex Luna C18 (250 mm × 4.6 mm, 5 μm) column with methanol-water (1: 9, V / V) at a flow rate of 0.5 mL / min using ultrasonic assisted extraction and reversed-phase high performance liquid chromatography The column temperature is room temperature; detection wavelength 224nm. The results showed that the linear relationship of allantoin between 1.596 and 7.182 μg was good with R 2 = 0.9995. The average recovery of allantoin was 97.2% and the RSD was 1.20% (n = 3). The stability, precision and recoveries of the method are good, which can provide reliable method for yam quality control. The content of allantoin in the yam of different producing areas is quite different. The content of allantoin in Nanyang yam is the highest and the content is the lowest Iron stick yam 124% higher.