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用简单缩聚法合成β-环糊精聚合物(β-CDP),并将其键合到自制二氧化硅微球上,合成新型的环糊精聚合物键合型手性固定相(CSP)。合成过程中考察了反应时间、原料比例对所制CSP的影响。用扫描电镜法检验了β-CDP键合硅胶圆整度,以四氮唑蓝法检测环糊精的含量为42.4μmol/g,其环糊精键合量明显高于β-CD单体键合硅胶。最后,将最优条件下得到的手性填料用高压匀浆法装柱,并进行液相色谱法拆分手性药物。将二者与ODS在相同的色谱条件下进行对比,结果表明,环糊精聚合物键合硅胶手性固定相对手性药物拆分能力更强。
Β-cyclodextrin polymer (β-CDP) was synthesized by a simple polycondensation method and bonded to self-made silica microspheres to synthesize a novel cyclodextrin polymer-bound chiral stationary phase (CSP) . During the synthesis process, the effects of reaction time and raw material ratio on the CSP were investigated. The roundness of β-CDP bonded silica gel was examined by scanning electron microscopy. The content of cyclodextrin was 42.4μmol / g by the method of tetrazolium blue assay, and the amount of cyclodextrin was significantly higher than that of β-CD monomer Combined silica gel. Finally, the chiral packing obtained under the optimal conditions is packed with high pressure homogenization method, and the chiral drugs are separated by liquid chromatography. Comparing the two with ODS under the same chromatographic conditions, the results show that the cyclodextrin polymer-bonded silica gel chiral stationary phase is more capable of resolving chiral drugs.