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Aim:To design the effective directed differentiation medium to differentiate bonemarrow cells into hepatocyte-like cells.Methods:Bone marrow cells were cul-tured in the directed differentiation media including fibroblast growth factor-4(FGF-4)and oncostatin M(OSM).Hepatocyte-like cells from directed differentia-tion of bone marrow cells were identified through cell morphology,RNA expres-sions by reverse transcriptase-polymerase chain reaction(RT-PCR),protein ex-pressions by Western blot,and hepatocellular synthesis and metabolism func-tions by albumin ELISA,Periodic acid-Shift staining and urea assay.Results:Some epithelial-like cells or polygonal cells appeared and increased in the courseof the cell directed differentiation.Hepatocyte nucleur factor-3[3(HNF-3β),albu-min(ALB),cytokeratin 18(CK18),transthyretin(TTR),glucose-6-phosphate(G-6-Pase),and tyrosine aminotransferase(TAT)mRNA were expressed in the courseof the directed differentiation.The directed differentiated cells on d 21 expressedHNF-3β,ALB,and CK18 proteins.The directed differentiated cells producedalbumin and synthesized urea in a time-dependent manner.They could also syn-thesize glycogen.Conclusion:Our differentiation media,including FGF-4 andOSM,are effective to differentiate bone marrow cells into hepatocyte-like cells,which could be used for hepatocyte resources for bioartificial liver or hepatocytetransplantation.
Aim: To design the effective directed differentiation medium to differentiate bonemarrow cells into hepatocyte-like cells. Methods: Bone marrow cells were cul-tured in the directed differentiation media including fibroblast growth factor-4 (FGF-4) and oncostatin M (OSM) . Hepatocyte-like cells from directed differentia-tion of bone marrow cells were identified through cell morphology, RNA expres-sions by reverse transcriptase-polymerase chain reaction (RT-PCR), protein ex-pressions by Western blot, and hepatocellular synthesis and metabolism func-tions by albumin ELISA, Periodic acid-shift staining and urea assay. Results: Some epithelial-like cells or polygonal cells have been increased in the course of the cell directed differentiation. Hepatatocyte nuclear factor-3 [HNF- albu-min (ALB), cytokeratin 18 (CK18), transthyretin (TTR), glucose- 6-phosphate (G-6-Pase), and tyrosine aminotransferase (TAT) mRNA were expressed in the course of the directed differentiation. cells on d 21 e xpressedHNF-3β, ALB, and CK18 proteins. The directed differentiated cells produced albumin and synthesized urea in a time-dependent manner.They could also syn-thesize glycogen. Conlusion: Our differentiation media, including FGF-4 and OSM, are effective to differentiate bone marrow cells into hepatocyte-like cells, which could be used for hepatocyte resources for bioartificial liver or hepatocyte transplant.