A.试剂1、饱和硫酸铵溶液煮沸溶解足够的(NH_4)_2SO_4,最少764克/升水放凉到室温。2、磷酸盐缓冲盐水(PBS),pH7.4。8.0克 NaCl0.2克 KH_2PO_42.9克 Na_2HPO_4.12H_2O0.2克 KCl0.2克 NaN_3将各种成分溶解在800毫升蒸馏水中,加到1,000毫升,核对 pH。3、吐温 PBS 液加1.0毫升吐温20到1,000毫升 PBS 液中, A. Reagents 1, saturated ammonium sulfate solution boil enough (NH 4) 2 SO 4, at least 764 g / L water to cool to room temperature. 2, Phosphate Buffered Saline (PBS), pH 7.4.8.0 g NaCl 0.2 g KH 2 PO 42.9 g Na 2 HPO 4.12 H 2 O 0.2 g KCl 0.2 g NaN 3 The ingredients were dissolved in 800 ml of distilled water and added to 1,000 ml Check the pH. 3, Tween PBS solution plus 1.0 ml Tween 20 to 1,000 ml PBS solution,