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目的 广西自1996年4月起在静脉吸毒者和卖血者中发现HIV感染者,为了解其传染来源和判断其流行趋势,对广西流行的HIV进行分子流行病学分析。方法 选取HIV抗体阳性血清标本44份,分别采用多肽酶免疫法(PEIA)和经逆转录PCR扩增作cDNA序列分析,分别确定其HIV-1基因亚型并加以比较。结果 结果表明广西存在4种HIV-1M组基因亚型,即B’(泰国B亚型)、C、D、E亚型。在静脉吸毒人群和性混乱者中存在HIV-1E亚型流行和C亚型感染者;而在卖血者中发现HIV-1B’和D亚型感染。结论 HIV-1D亚型感染和E亚型流行已在国内出现,E亚型病毒已由东南亚传入流行并将在我国南部形成新的流行区域。提出血清学分型方法可作为HIV-1基因亚型分析的筛选技术推广应用。
Objective Guangxi has found HIV-infected persons among intravenous drug users and blood donors since April 1996. To understand its source of infection and determine its epidemic trend, molecular epidemiological analysis of HIV prevalence in Guangxi was conducted. Methods Forty-four HIV seropositive HIV serogroups were selected and their HIV-1 genotypes were determined by polymerase chain immunoassay (PEIA) and reverse transcriptase PCR (PCR), respectively. The results showed that there are four HIV-1M genomic subtypes in Guangxi, namely B ’(Thai B subtype), C, D, E subtypes. HIV-1E and C subtypes are present in intravenous and sexual confounders; HIV-1B ’and D subtypes are found in blood donors. Conclusion HIV-1D subtypes and subtypes of E-subtype have been reported in China. E-subtype A virus has been introduced from Southeast Asia and will form a new epidemic region in southern China. It is suggested that the serological typing method can be used as a screening technology of HIV-1 subtype analysis.