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危害柞蚕(Antheraea pernyi)、栗蚕(Dictyoploca japonica)等野蚕的寄生蝇种类较多,准确鉴定其种类是害虫防治的基础.对采集自不同年份(2007年和2016年)、不同地理区域(辽宁、吉林和河南)寄生柞蚕和栗蚕的50份寄生蝇蛹样品的DNA条形编码进行了研究.来自辽宁6个地区和吉林2个地区的38份柞蚕寄生蝇样品属于柞蚕饰腹寄蝇(Blepharipatibialis),它们的DNA条形编码序列的碱基一致性达100％,表明辽宁和吉林的柞蚕饰腹寄蝇均来源于一个扩散点,而且扩散历史极短.来自河南的柞蚕寄生蝇样品HN6的DNA条形编码序列与家蚕追寄蝇(Exorista sorbillans)的序列一致性达到96％,属于家蚕追寄蝇;而另外的5份样品HN1～5的序列一致性为100％,推测属于蚕饰腹寄蝇(Blepharipa zebina).来自辽宁的寄生栗蚕的6份寄生蝇样品DJY1～6的DNA条形编码序列完全一致,但其分类学地位有待于进一步确认.有趣的是,来自河南同一个柞蚕茧内的寄生蝇样品HN3～5和HN6分属于2种寄生蝇.上述结果表明,利用DNA条形编码技术进行危害野蚕的寄生蝇种类鉴定和种群发生监测是可行的.“,”Wild silkworms like Antheraea pernyi and Dictyoploca japonica can be parasitized by a variety of tachinids.Species identification of these tachinids plays a basic role for their control.To address this issue,the present study compared the DNA barcode sequences of 50 tachinid pupa samples collected from three geographic regions (Liaoning,Jilin,and He'nan provinces) in 2007 and 2016 in parasitized A.pernyi and D.japonica.Thirty-eight samples of A.pernyi tachinid from Liaoning and Jilin provinces exhibited entire identical DNA barcode sequences.This tachinid was further identified to be Blepharipa tibialis,thus indicating that they were derived from a single dispersal locus with a very short dispersal history.One A.pernyi tachinid sample (i.e.HN6) from He'nan Province was identified as Exorista sorbillans based on 96％ sequence identity of DNA barcode between them,whereas the other five samples (i.e.HN1-5) with identical DNA barcode sequence was supposed to be Blepharipa zebina.Six D.japonica tachinid samples (i.e.DJY1-6) also exhibited identical DNA barcode sequences,but their taxonomic status remained unclear.It's amazing that two different tachinid species can simultaneously parasitize one individual of A.pernyi silkworm larva as shown in a sample from He'nan.This study suggests that it is feasible for fast species identification and timely population dynamics monitoring of tachinids parasitic to wild silkworms through DNA barcode.