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目的:建立人血浆去氧氟尿苷检测的高效液相色谱方法。方法:血浆经高氯酸沉淀,以Venusil MP-C18为色谱柱;流动相为乙腈-水-0.1%三氟醋酸体系,流速为0.8mL·min-1;检测波长为266nm。结果:去氧氟尿苷在0.10～10.00mg·L-1范围内线性关系良好(r=0.9999);定量下限为0.10mg·L-1;高、中、低3个浓度的相对回收率分别为(98.4±3.3)%,(102.9±1.5)%,(100.5±1.0)%;日内RSD分别为3.15%,1.52%,1.03%,日间RSD分别为5.61%,3.54%,1.65%。结论:本方法准确可靠、简便快速,适用于人血浆去氧氟尿苷浓度的测定及其药动学研究。
Objective: To establish a HPLC method for the determination of doxifluridine in human plasma. Methods: The plasma was precipitated by perchloric acid, and Venusil MP-C18 was used as the column. The mobile phase consisted of acetonitrile-water-0.1% trifluoroacetic acid and the flow rate was 0.8 mL · min-1. The detection wavelength was 266 nm. Results: The calibration curve of doxifluridine was linear in the range of 0.10-10.00 mg · L-1 (r = 0.9999). The lower limit of quantitation was 0.10 mg · L-1. The relative recoveries of high, medium and low concentrations were Were (98.4 ± 3.3)%, (102.9 ± 1.5)% and (100.5 ± 1.0)%, respectively. The intraday RSD were 3.15%, 1.52% and 1.03% respectively. The daytime RSD were 5.61%, 3.54% and 1.65% respectively. Conclusion: The method is accurate and reliable, simple and rapid, suitable for the determination of the plasma concentration of doxifluridine and its pharmacokinetic study.