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二乙基亚硝胺诱发实验性肝癌过程中,用电镜观察肝结节内肝细胞及G-6-Pase的改变,结果显示:(1)肝细胞问的连接较正常为少,部分区域的细胞连接消失,细胞问隙增宽。(2)部分肝细胞核有凹陷,核仁肿大,胞质内线粒体嵴变短而少,粗面内质网膜上核糖体脱落,糖原颗粒减少。(3)少数细胞的细胞核增大,胞质内游离核糖体丰富,各细胞器不发达。(4)诱癌第8周,肝细胞内G-6-Pase呈阳性反应,12周后为阴性。这些特点可认为是肝细胞去分化的主要指示。
In the course of experimental hepatocarcinogenesis induced by diethylnitrosamine, the changes of hepatocytes and G-6-Pase in liver nodules were observed by electron microscopy. The results showed that: (1) The connection of hepatocytes was less than normal, and some areas were The cell connection disappeared and the cell gap widens. (2) Some hepatic cell nuclei have sag, nucleolar enlargement, mitochondria in the cytoplasm become shorter and less, ribosomes on the rough endoplasmic reticulum fall off, and glycogen granules decrease. (3) The nucleus of a few cells increases, the cytoplasm is rich in free ribosomes, and each organelle is underdeveloped. (4) At the 8th week after induction of cancer, G-6-Pase was positive in hepatocytes and negative after 12 weeks. These characteristics can be considered as the main indicators of dedifferentiation of hepatocytes.