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为了对中药复方免疫增强剂中的多糖进行纯化,试验采用苯酚硫酸法测定多糖含量,然后以AB-8大孔吸附树脂为分离材料,以乙醇为洗脱剂,系统研究上样液浓度、上样量、上样速率、洗脱剂乙醇浓度、洗脱速率、洗脱剂用量等参数对多糖吸附-解吸效果的影响。结果表明:上样液浓度为5.93 mg/mL,上样量为1倍量树脂体积(BV),上样液速率为每小时2倍树脂体积数;洗脱剂乙醇浓度为50%,洗脱速率为每小时3倍树脂体积数,洗脱剂用量为3倍量树脂体积;吸附率为74.3%,解吸率为93.6%。说明AB-8大孔树脂可用于纯化中药复方免疫增强剂中的多糖。
In order to purify the polysaccharides in traditional Chinese medicine compound immunostimulants, the content of polysaccharides was determined by the phenol-sulfuric acid method. Then the AB-8 macroporous resin was used as the separation material and the ethanol as the eluent to systematically study the concentration of the sample solution. Sample size, loading rate, eluent ethanol concentration, elution rate, eluent dosage and other parameters on polysaccharide adsorption - desorption effect. The results showed that the sample volume was 5.93 mg / mL, the sample volume was 1 × resin volume (BV), the sample volume was 2 × resin volume per hour, and the eluent ethanol concentration was 50% The rate is 3 times the volume of resin per hour, the amount of eluent is 3 times the volume of resin; the adsorption rate is 74.3% and the desorption rate is 93.6%. Description AB-8 macroporous resin can be used to purify polysaccharides in traditional Chinese medicine compound immunostimulants.