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目的探讨前列腺素E_2（PGE_2）在膀胱癌患者淋巴因子激活的杀伤细胞（LAK）增殖及细胞毒的作用。方法LAK细胞培养于含不同浓度的PGE_2；的培养基中，并用细胞计数法测定其细胞增殖率，以BIU87、EJ及患者自体肿瘤细胞为靶细胞，用MTT法测定LAK细胞对膀胱癌细胞的细胞毒作用。结果0.05～5μg／L。PGE_2对LAK细胞的增殖呈浓度依赖性抑制。PGE_2LAK细胞对膀胱癌细胞的杀伤则影响不明显。膀胱癌细胞系BIU87的条件培养基的PGE_2含量明显高于PBMC的条件培养基。结论膀胱癌患者由IL－2诱导的LAK增殖可被由PBMC和膀胱癌细胞产生的PGE_2所抑制。
Objective To investigate the effects of prostaglandin E_2 (PGE_2) on proliferation and cytotoxicity of lymphokine-activated killer (LAK) cells in patients with bladder cancer. Methods LAK cells were cultured in medium containing different concentrations of PGE 2; cell proliferation rate was measured by cell counting. BIU87, EJ and autologous tumor cells were used as target cells. MTT was used to determine the effect of LAK cells on bladder cancer cells Cytotoxic effects. Results 0.05 ~ 5μg / L. PGE 2 inhibited LAK cell proliferation in a concentration-dependent manner. PGE_2LAK cells on bladder cancer cell killing effect is not obvious. The PGE 2 content of conditioned medium of bladder cancer cell line BIU87 was significantly higher than that of PBMC conditioned medium. Conclusions LAK proliferation induced by IL-2 in bladder cancer patients can be suppressed by PGE 2 produced by PBMCs and bladder cancer cells.