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采用同位素测定法分别对急性和慢性白血病患者血浆环腺苷一磷酸(cAMP)、腺苷酸环化酶(AC)和环腺苷酸磷酸二酯酶(cAPDE)三项指标进行了检测,并与非白血病患者和正常献血员进行比较,发现白血病组的cAMP水平分别为10．23±1．64和12．57±1．38pmol/ml,较正常对照组29．78±2.66pmol/m显著降低(P<0．01),AC比活力分别为0．32±0．02和0．44±0．06U/mg蛋白质,较正常对照组0．65±0．06U/mg蛋白质亦显著降低(P<0．01),而cAPDE比活力较正常对照组无明显差异(P>0.05).在非白血病患者组,上述指标较正常对照组的差异无统计学意义(P>0．05)。提示白血病患者cAMP水平降低,其原因是由于AC比活力下降的结果,而与cAPDE比活力的变化无明显相关性。
Plasma levels of cAMP, adenylyl cyclase (AC), and cAMP (cAMPDE) were measured using isotope assays in acute and chronic leukemia patients. Compared with non-leukemia patients and normal blood donors, the cAMP levels in the leukemia group were 10.23±1.64 and 12.57±1.38 pmol/ml, respectively, which were significantly greater than the normal control group at 29.78±2.66 pmol/m. With a decrease (P<0.01), the specific AC activity was 0.32±0.02 and 0.44±0.06 U/mg protein, respectively, which was significantly lower than that of the normal control group with 0.65±0.06 U/mg protein. (P<0.01), but the specific activity of cAPDE was not significantly different from the normal control group (P>0.05). In the non-leukemia patient group, the above-mentioned indicators were not significantly different from the normal control group (P>0.05). . It is suggested that the decrease of cAMP level in leukemia patients is due to the decrease of AC specific activity, but no significant correlation with the change in specific activity of cAPDE.