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目的通过对2013至2014年西宁地区5岁以下儿童病毒性腹泻监测数据分析掌握其流行现状。方法收集青海省儿童医院,青海省红十字医院2013年至2014年5岁以下腹泻门诊患儿粪便标本191份,采用多重,巢式PCR方法同时进行轮状病毒Rotavirus(RV),诺如病毒Norovirus(NV),星状病毒astrovirus(ASTV),扎如病毒sapovirus(SAV)和肠道腺病毒adenovirus(EADV)核酸检测。结果 191份粪便标本中,5种病毒感染检出阳性率分别为34.55%,8.38%,2.62%,4.71%,2.62%,96份样本至少检出一种腹泻病毒核酸,其中91份检出单一病毒核酸,5份是双重感染。结论2013至2014年西宁市5岁以下儿童的RV检测阳性率最高,NV次之,SAV在次,ASTV和EADV阳性率最低,另外还有混合感染现象。今后应加强婴幼儿腹泻监测,并关注混合感染。
Objective To analyze the epidemiological status of viral diarrhea in children under 5 in Xining during 2013-2014. Methods Totally 191 stool specimens from children with diarrhea outpatients under 5 years of age from 2013 to 2014 in Qinghai Provincial Children’s Hospital and Qinghai Red Cross Hospital were collected. Rotavirus (RV), Norovirus Norovirus (NV), astrovirus (ASTV), Zapovirus (SAV) and enterovirus adenovirus (EADV) nucleic acids. Results Among the 191 stool samples, the positive rates of the five virus infections were 34.55%, 8.38%, 2.62%, 4.71% and 2.62%, respectively. A total of 96 samples were detected for at least one diarrhea virus nucleic acid, of which 91 were single Virus nucleic acid, 5 copies are double infection. Conclusion The positive rate of RV test was the highest among children under 5 years of age in Xining from 2013 to 2014, followed by NV, followed by SAV, with the lowest positive rates of ASTV and EADV and the mixed infection. In the future, we should strengthen the monitoring of infantile diarrhea and pay attention to mixed infection.