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目的:研究不同类型牙周炎患者牙龈组织中IL-21基因的表达,探讨其在牙周炎发病中的作用。方法:选择慢性牙周炎患者12例,侵袭性牙周炎8例,健康对照组8例,采用实时定量PCR方法定量检测IL-21 m RNA在牙龈组织表达情况。结果:慢性牙周炎、侵袭性牙周炎、健康对照组牙龈组织中均有IL-21 m RNA的表达,慢性牙周炎组和侵袭性牙周炎组IL-21 m RNA相对表达量分别为0.000534±0.000504和0.00602±0.000137,显著高于健康对照组0.000161±0.000352,差异有统计学意义(P<0.05)。慢性牙周炎和侵袭性牙周炎牙龈组织IL-21m RNA表达没有显著性差异(P>0.05)。结论:IL-21在慢性牙周炎发病机制中可能发挥重要作用。
Objective: To study the expression of IL-21 gene in gingival tissues of different types of periodontitis and to explore its role in the pathogenesis of periodontitis. Methods: Twelve patients with chronic periodontitis, eight patients with aggressive periodontitis and eight healthy controls were enrolled in this study. Quantitative real-time PCR was used to detect the expression of IL-21 mRNA in gingival tissues. Results: The expressions of IL-21m RNA in gingival tissues of chronic periodontitis, aggressive periodontitis and healthy control group were both higher than those of chronic periodontitis group and aggressive periodontitis group 0.000534 ± 0.000504 and 0.00602 ± 0.000137 respectively, which was significantly higher than 0.000161 ± 0.000352 in healthy controls (P <0.05). There was no significant difference in IL-21m RNA expression between gingival tissues and chronic periodontitis and aggressive periodontitis (P> 0.05). Conclusion: IL-21 may play an important role in the pathogenesis of chronic periodontitis.