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构建编码HBV包膜 核心蛋白融合基因的DNA疫苗pSC、pSS1S2C和编码HBV包膜蛋白或核心蛋白基因的DNA疫苗 pHBs、pHBc ,分别肌肉注射免疫BALB/c小鼠 ,检测小鼠的血清抗体、T细胞增殖和细胞毒性T淋巴细胞反应 ,比较融合基因DNA疫苗与单基因DNA疫苗诱生免疫应答的强度 ,发现融合基因DNA疫苗诱生抗体的效率明显不及单基因DNA疫苗 ,但其能诱导更强、更持久的细胞免疫应答 ,表明HBV包膜 核心蛋白融合基因DNA疫苗对于治疗慢性乙型肝炎可能比单基因DNA疫苗更为有效 .
To construct DNA vaccine pSC, pSS1S2C and DNA vaccine pHBs and pHBc which encode HBV envelope protein or core protein gene respectively, BALB / c mice were immunized intramuscularly to detect the serum antibody, T Cell proliferation and cytotoxic T lymphocyte response, the fusion DNA vaccine and single-gene DNA vaccine induced immune response strength and found that the fusion gene DNA vaccine induced antibody efficiency was significantly less than single-gene DNA vaccine, but it can induce stronger , A more durable cellular immune response, suggesting that HBV envelope fusion protein DNA vaccines may be more effective than single-gene DNA vaccines in the treatment of chronic hepatitis B.