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中医认为温热病邪,灼伤津血,津液不足,不能载血运行。血受薰灼,则易于凝结瘀塞,导致血瘀。温病是外感六淫中具有热象特点的一类疾病。本实验就用绿脓杆菌血行感染,造成血与热结的热毒血瘀证模型。现报告如下: 实验材料与方法 (一)菌种:经琼酯培养基转种14小时的绿脓杆菌菌落,生理盐水洗脱。以1％BaCl_2和1％H_2SO_4配成梯度比浊管,用721分光光度计在λ=520nm处测OD值,制作标准曲线,并经回归方程检验。再将洗脱菌落液测OD值,从标准曲线得知所含菌数,再稀释成1亿/ml绿脓杆菌菌液备用。
Chinese medicine believes that the disease is caused by a fever, fever, and blood deficiency, and that the body fluid is insufficient and cannot be carried by blood. If the blood is burned, it will be easy to clot and cause bloody sputum. Warm disease is a type of disease characterized by thermal image in exogenous sex. In this experiment, Pseudomonas aeruginosa was used to infect the blood, resulting in a blood-heat and blood-heat syndrome. The report is as follows: Experimental Materials and Methods (I) Bacteria: Pseudomonas aeruginosa colonies that have been transferred to agar medium for 14 hours and eluted with physiological saline. A grading turbidity tube was set up with 1% BaCl 2 and 1% H 2 SO 4 , and the OD value was measured at λ = 520 nm with a 721 spectrophotometer to make a standard curve and examined by a regression equation. The OD value of the colony was measured by elution, and the number of contained bacteria was determined from the standard curve, and diluted again to 100 million/ml of the bacillus bacillus solution.