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一氧化氮(NO)不仅作为血管内皮依赖性舒张因子(EDRF)调节血管张力,在血管舒张起重要作用,而且是机体细胞间一种新型的信息分子,NO 与血压的调节,神经传导及宿主的防御能力都有着极其密切的关系。本文采用荧光分光光度法,测定正常妊娠的血浆 NO 含量,以探讨 NO在正常妊娠过程中的生理作用。1 材料和方法①标本收集:对照组15例未孕妇女,未服避孕药。无心、肝、肾脏及免疫病史,正常妊娠组30例,无产科并发症和内科合并症。晨6～8时空腹取肘前静脉血5ml,1h 内4℃2000rpm/min 离心10min,分离后血浆于-20℃
Nitric oxide (NO) not only regulates vascular tone as endothelium-dependent relaxing factor (EDRF), plays an important role in vasodilatation, but also is a new type of information molecule among body cells, regulating NO with blood pressure, nerve conduction and host Defense capabilities have a very close relationship. In this paper, fluorescence spectrophotometry was used to determine plasma NO level in normal pregnancy to explore the physiological role of NO in normal pregnancy. 1 Materials and Methods ① specimens collected: 15 cases of non-pregnant women control group, did not take birth control pills. No heart, liver, kidney and immune history, normal pregnancy group of 30 cases, no obstetric complications and medical complications. Morning 6 ~ 8 fasting antelectasis 5ml venous blood, centrifuged 10min at 4 ℃ 2000rpm / min within 1h, after separation plasma at -20 ℃