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目的:建立番石榴叶提取物中金丝桃苷、异槲皮苷、瑞诺苷、槲皮素-3-O-β-D-吡喃阿拉伯糖苷、槲皮素-3-O-α-L-呋喃阿拉伯糖苷的多成分的含量测定方法。方法:色谱柱:S y n c r o n i s C18色谱柱(4.6mm×250mm,5μm),以乙腈-0.2%磷酸水梯度洗脱,流速1.0mL/min,检测波长254nm,柱温40℃。结果:金丝桃苷、异槲皮苷、瑞诺苷、槲皮素-3-O-β-D-吡喃阿拉伯糖苷、槲皮素-3-O-α-L-呋喃阿拉伯糖苷的线性范围为0.0318-0.2544μg(r=0.9998)、0.0575-0.4600μg(r=0.9998)、0.1860-1.4880μg(r=0.9998)、0.1482-1.1856μg(r=0.9998)、0.2012-1.6816μg(r=0.9998),平均加样回收率分别为100.7(RSD=2.6%),100.5(RSD=0.8%),103.4(RSD=2.6%),98.1(RSD=1.7%),100.7(RSD=1.2%),精密度、重复性、稳定性均良好。结论:所建立的方法准确可靠,重复性好,可用于番石榴叶提取物的含量测定。
OBJECTIVE: To establish a method for the determination of hyperoside, isoquercetin, glycoside, quercetin-3-O-β-D-arabinopyranoside, quercetin-3-O- Method for the determination of multi-component content of L-arabinofuranoside. METHODS: The column: S yn c r o n i s C18 column (4.6 mm × 250 mm, 5 μm) eluted with a gradient of acetonitrile-0.2% phosphoric acid with a flow rate of 1.0 mL / min and a detection wavelength of 254 nm at a column temperature of 40 ° C. RESULTS: The linearity of hyperoside, isoquercitrin, linoleoside, quercetin-3-O-β-D-arabinopyranoside, quercetin-3-O-α-L-arabinofuranoside (R = 0.9998), 0.1482-1.1856μg (r = 0.9998), 0.2012-1.6816μg (r = 0.9998), 0.1882-1.4880μg (r = 0.9998) ), And the average recoveries were 100.7 (RSD = 2.6%), 100.5 (RSD = 0.8%), 103.4 (RSD = 2.6%), 98.1 (RSD = 1.7%) and 100.7 Degree, repeatability, stability are good. Conclusion: The established method is accurate and reproducible, and can be used for the determination of the content of guava leaf extract.