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目的探讨氢醌(HQ)对体外培养人支气管上皮细胞DNA损伤及细胞周期的影响。方法将HBE细胞用不同浓度(0、5、10、20、40、80、160、320μmo/lL)的氢醌作用24 h,采用噻唑蓝(MTT)比色法测定16HBE细胞的相对存活率,用单细胞凝胶电泳(SCGE)检测细胞DNA的损伤情况,流式细胞术检测细胞周期分布。结果在0～40μmo/lL范围内HQ作用24 h后,16HBE细胞的存活率未见明显变化(P>0.05);当染毒剂量超过80μmo/lL时,细胞存活率明显下降(P<0.01)。SCGE显示随着HQ浓度的升高,16HBE细胞的DNA断裂程度加重。HQ作用浓度在10～320μmo/lL范围内,16HBE细胞的细胞周期表现为G2期阻滞,G1期比例下降。结论 HQ会对16HBE细胞的DNA产生损害,并且引起G2期细胞阻滞。
Objective To investigate the effect of hydroquinone (HQ) on DNA damage and cell cycle in cultured human bronchial epithelial cells. Methods The HBE cells were treated with different concentrations of hydroquinone (0, 5, 10, 20, 20, 40, 80, 160 and 320μmo / L) for 24 h. The relative viability of 16HBE cells was determined by MTT assay. Cell DNA damage was detected by single cell gel electrophoresis (SCGE), and cell cycle distribution was analyzed by flow cytometry. Results The survival rate of 16HBE cells did not change significantly (P> 0.05) after treated with HQ for 24 h in the range of 0-40 μmol / L, and significantly decreased (P <0.01) when the dose was over 80 μmol / . SCGE showed that the degree of DNA fragmentation in 16HBE cells increased with the increase of HQ concentration. HQ concentration in the range of 10 ~ 320μmo / lL, 16HBE cell cycle showed G2 arrest, the proportion of G1 decreased. Conclusion HQ can damage the DNA of 16HBE cells and cause cell arrest in G2 phase.