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本文旨在根据前期研究建立的恒河猴感染动物模型,对同期研制的肠道病毒71型(EV71)实验性灭活疫苗免疫动物进行全面的免疫保护性评价。评价指标包括病毒攻击后动物体内病毒载量及病理学变化,根据所得结果进行实验性疫苗免疫后动物在病毒攻击中的感染动力学分析。3个疫苗剂量(20、80、320EU)免疫的恒河猴均出现不同效价的中和抗体,80EU和320EU剂量组在二次免疫后第6周抗体效价达1∶128～1∶256,经104.5CCID50病毒鼻腔攻击后均未检出阳性病毒载量。20EU剂量组中,淋巴器官、中枢神经系统及其他主要脏器均出现比对照组低但仍为阳性的病毒增殖现象。病理学方面,各剂量组免疫恒河猴的中枢神经系统以及肺等器官均未出现相关病理损伤。本实验在确定该疫苗对恒河猴有效保护性的同时,亦为明确EV71灭活疫苗免疫剂量提供了直接的实验依据。
The aim of this paper is to evaluate the immunoprotective effects of the experimentally inactivated EV71 inactivated vaccine against the rhesus monkeys infected animal model established in the previous study. The evaluation index includes the viral load and pathological changes in the animal after virus challenge, and according to the obtained results, the dynamic analysis of the infection in the animal after the virus is challenged by the experimental vaccine is carried out. Antibody titer of all the rhesus monkeys immunized with 3 doses of vaccine (20,80,320EU) showed different potency. The antibody titer in the 80EU and 320EU groups was 1:128 ~ 1:256 at the 6th week after the second immunization , No positive viral load was detected after 104.5CCID50 virus nasal attack. 20EU dose group, lymphoid organs, the central nervous system and other major organs appear lower than the control group but still positive virus proliferation phenomenon. Pathology, the immune system of each dose of rhesus monkey and other organs of the lungs and other pathological injury did not appear. This experiment in determining the effective protection of the vaccine on rhesus monkeys, but also to clarify the immunization dose of EV71 inactivated vaccine provides a direct experimental basis.