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目的比较荧光原位杂交法(FISH)与免疫组化法(IHC)检测乳腺癌组织中Her2基因扩增和蛋白表达的差异性和相关性。方法IHC法检测56例乳腺癌细胞膜上Her2蛋白表达情况,FISH法检测细胞核内Her2基因扩增情况,χ2检验及kappa检验对两种方法检测结果进行统计分析。结果两种方法的检测Her2阳性率差异有统计学意义(χ2=39.867,P<0.05);IHC法检测Her2蛋白0～1+与3+之间,通过FISH法检测Her2基因扩增的一致性好(k=0.906,P<0.05);而IHC检测Her2蛋白2+与3+之间,FISH检测Her2基因扩增一致性较差(k=0.357,P<0.05)。结论IHC检测Her2蛋白为2+的需做FISH检测,以明确Her2基因扩增的情况,FISH检测法提高了诊断的准确性和特异性。
Objective To compare the difference and correlation of Her2 gene amplification and protein expression in breast cancer by fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC). Methods The expression of Her2 protein in 56 cases of breast cancer cell membrane was detected by IHC method. The amplification of Her2 gene in nucleus was detected by FISH method. The results ofχ2 test and kappa test were used to analyze the results of two methods. Results The positive rates of Her2 in the two methods were significantly different (χ2 = 39.867, P <0.05). Her2 protein was detected by IHC between 0 ~ 1 + and 3+, and the consistency of Her2 gene amplification was detected by FISH (K = 0.906, P <0.05). However, Her2 protein between 2 and 3+ was detected by IHC, and Her2 gene amplified by FISH was less consistent (k = 0.357, P <0.05). Conclusions The detection of Her2 protein in IHC by IHC needs to be performed by FISH to confirm the amplification of Her2 gene. The accuracy and specificity of the FISH method for diagnosis are improved.