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背景:稳定准确的动物模型是研究出血性脑血管病的必要工具和基础。目的:建立和评价大鼠脑内囊出血模型。设计:随机对照动物实验。单位:徐州医学院第二附属医院;南京医科大学第一附属医院。材料:实验于2002-05/11在南京医科大学动物实验中心完成。35只SD大鼠随机分为两组:实验组30只,假手术组5只。方法:①通过立体定向术向实验组大鼠脑内囊注入自体血制成脑内囊出血模型。②按ZeaLonga5分制神经病学评分标准评分,观察大鼠躯体感觉及运动功能。③大鼠在麻醉状态下于术前及术后检测体感诱发电位。④测定体感诱发电位后,将大鼠麻醉后处死,取脑制备切片,苏木精-伊红染色,以最大病灶处光镜观察血肿及组织形态学的改变。主要观察指标:①两组大鼠神经功能评分。②两组大鼠体感诱发电位各波潜伏期。③两组大鼠脑组织形态学观察。结果:35只大鼠均进入结果分析。①以出现明显偏瘫为造模成功,本实验成功率为93.3%(28/30),实验组神经病学评分为(2.74±0.46)分,与假手术组(0分)比较,差异有显著性(P<0.05)。②体感诱发电位显示,实验组术后各波潜伏期较术前和假手术组明显延迟[P1:(15.72±0.78)ms,(10.69±0.52)ms,(10.73±0.48)ms;N1:(17.95±1.27)ms,(13.21±1.31)ms,(13.34±1.27)ms;N2:(21.16±1.62)ms,(15.42±1.46)ms,(15.58±1.44)ms;N3:(24.86±1.58)ms,(18.72±1.76)ms,(18.99±1.67)ms,P<0.05]。③假手术组仅见针道周围散在红细胞,无出血灶;实验组病理形态学表现为左侧内囊区不规则或椭圆形血凝块,大致有一个低倍视野范围,出血灶边缘脑组织疏松水肿,病变明显重于假手术组。结论:用立体定向术回注自体血制成大鼠脑内囊出血模型更接近临床脑出血,且方法简便,重复性好。
Background: Stable and accurate animal models are necessary tools and basis for the study of hemorrhagic cerebrovascular disease. Objective: To establish and evaluate a model of intracerebral hemorrhage in rats. Design: Randomized controlled animal experiments. Unit: Second Affiliated Hospital of Xuzhou Medical College; First Affiliated Hospital of Nanjing Medical University. Materials: The experiment was performed at Animal Experimental Center, Nanjing Medical University from May to May 2002. Thirty-five SD rats were randomly divided into two groups: 30 in experimental group and 5 in sham-operated group. Methods: ① Intrathecal injection of autologous blood into the intracerebral cysts of rats in experimental group by stereotaxic surgery was made into a model of intracerebral hemorrhage. ② According to ZeaLonga5 score neurological score standard score to observe the somatosensory and motor function of rats. ③ rats under anesthesia in preoperative and postoperative somatosensory evoked potentials. After determining somatosensory evoked potentials, the rats were killed after anesthesia, the brain was prepared and stained with hematoxylin-eosin, the changes of hematoma and histomorphology were observed under light microscope with the largest lesion. MAIN OUTCOME MEASURES: ①Nervous function score of two groups of rats. ② two groups of somatosensory evoked potentials of each wave latency. ③ Morphology of brain in both groups. Results: All 35 rats were involved in the result analysis. (1) The success rate of the experiment was 93.3% (28/30), the neurological score of the experimental group was (2.74 ± 0.46) points, compared with the sham operation group (0 point), the difference was significant (P <0.05). The somatosensory evoked potentials showed that the latencies of each wave in the experimental group were significantly delayed compared with those in the preoperative and postoperative groups [P1: (15.72 ± 0.78) ms, (10.69 ± 0.52) ms, (10.73 ± 0.48) ms; N1: ± 1.27) ms, (13.21 ± 1.31) ms, (13.34 ± 1.27) ms; N2: (21.16 ± 1.62) ms, (15.42 ± 1.46) ms, (15.58 ± 1.44) ms; , (18.72 ± 1.76) ms, (18.99 ± 1.67) ms, P <0.05]. ③ In the sham-operated group, only the red blood cells scattered around the needle track and no hemorrhage were found in the sham-operated group. The histopathology in the experimental group showed irregular or oval blood clots in the left internal capsule area. Edema, lesions significantly heavier than the sham group. Conclusion: The model of intracerebral hemorrhage in rats with stereotactic reinfusion of autologous blood is closer to clinical cerebral hemorrhage. The method is simple and reproducible.