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目的:建立HPLC测定藏族药圆柏和刺柏中3种主要黄酮类成分的方法。方法:采用HPLC,样品经70%甲醇超声,采用CAPCELL PAK C18色谱柱(4.6 mm×250 mm,5μm),流动相0.2%磷酸乙腈-0.2%磷酸水梯度洗脱,流速1.0 m L·min-1,检测波长360 nm,柱温40℃。结果:芦丁在0.004 9~0.146 4μg(r=0.999 9);槲皮苷在0.009 4~0.281 4μg(r=0.999 8);山柰酚在0.005 4~0.162 0μg(r=0.999 8)进样量与峰面积均成良好的线性关系;芦丁、槲皮苷和山柰酚的加样回收率分别为100.12%(RSD 2.6%),96.35%(RSD 0.7%),96.13%(RSD 0.5%)。结论:3种黄酮在40 min内达到基线分离。该方法分析时间短、稳定性和准确度良好,对圆柏和刺柏药材的质量控制具有一定的参考价值。
OBJECTIVE: To establish a method for the determination of three major flavonoid components in Tibetan medicinal materials Sabina and Juniperus. Methods: The samples were eluted with 70% methanol by CAPCELL PAK C18 column (4.6 mm × 250 mm, 5 μm) and mobile phase consisted of 0.2% phosphoric acid and 0.2% phosphoric acid. The flow rate was 1.0 m L · min- 1, detection wavelength 360 nm, column temperature 40 ℃. Results: Rutin was 0.004 9 ~ 0.146 4 μg (r = 0.999 9), quercetin was 0.009 4 ~ 0.281 4 μg (r = 0.999 8), and kaempferol was injected with 0.005 4 ~ 0.162 0 μg (r = 0.999 8) The recoveries of rutin, quercitrin and kaempferol were 100.12% (RSD 2.6%), 96.35% (RSD 0.7%) and 96.13% (RSD 0.5%), respectively. ). Conclusion: The three flavonoids achieve baseline separation within 40 min. The method has the advantages of short analysis time, good stability and accuracy, and has certain reference value for the quality control of Sabina przewalskii and Sabina vulgaris.