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本文作者采用流式细胞术对51例食管癌手术患者上下切缘和癌灶组织147个样品的DNA倍体进行了分析。结果表明,切缘和癌灶均出现了DNA异常倍体,其检出率分别为50.0%、63.0%和70.6%,均以非整倍体为主。上下切缘的DNA指数明显低于癌灶(P<0.01)。超二倍体细胞比率和S期细胞百分比,各组均高于正常粘膜组(P<0.01),而各组间均无显著差异(P>0.05)。
The authors used flow cytometry to analyze DNA ploidy in 147 samples of the upper and lower resection margins and cancer foci in 51 patients with esophageal cancer surgery. The results showed that the DNA abnormity occurred in both margin and foci, and the detection rates were 50.0%, 63.0% and 70.6%, respectively, and all were aneuploidy. The DNA index of the upper and lower margins was significantly lower than that of the foci (P<0.01). The ratio of hyperdiploid cells and percentage of cells in S phase was higher in each group than in normal mucosa (P<0.01), but there was no significant difference among the groups (P>0.05).