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【目的】通过分析毛棉苗期抗旱相关性状的数量性状位点(Quantitative trait locus,QTL),以期检测稳定的主效QTL,促进栽培品种抗旱性状遗传改良及提高抗旱育种效率。【方法】以四倍体野生种毛棉(Gossypium tomentosum)和陆地棉品种中棉所12(CCRI 12)的种间杂种F2及其F2:3家系为研究材料,用于基因型分型的F2有188个系,用于表型分型的F2:3家系有149个株系。分别在干旱胁迫和正常灌水2个环境下调查表型数据。采用复合区间作图法对F2:3家系苗期相关性状抗旱系数进行QTL定位。【结果】对苗期相关性状抗旱系数的QTL定位分析,共得到16个QTL,其中与株高、叶片数、叶绿素含量、脯氨酸含量、丙二醛含量抗旱系数相关的QTL分别有5个、1个、3个、3个、4个,分布在13条染色体上。来自毛棉的5个加性QTL分别为qSHDC-19-1、qSHDC-19-2、qSLNDC-5-1、qMDADC-24-1、qMDADC-24-2,其加性效应值为0.10~0.22,解释变异9.4%~25.8%。【结论】这些与抗旱相关的QTL有助于棉花抗旱分子标记辅助选择。
【Objective】 Quantitative trait locus (QTL) for drought-related traits in wool and cotton seedling stage was used to detect stable major trait loci (QTL) and to promote genetic improvement of drought-tolerant traits in cultivars and to improve drought-resistant breeding efficiency. 【Method】 The interspecific hybrid F2 and F2: 3 pedigree of tetraploid wild-type Gossypium tomentosum and CCRI 12 were used as genotypes for F2 There are 188 lines, F2: 3 lines for phenotyping have 149 lines. Phenotypic data were investigated under two environments of drought stress and normal irrigation respectively. The QTL mapping of the drought resistance coefficient of F2: 3 lineage-related traits at seedling stage was performed by composite interval mapping. 【Result】 A total of 16 QTLs were obtained for QTL mapping of drought resistance related traits at seedling stage. Among them, 5 QTLs related to drought resistance of plant height, leaf number, chlorophyll content, proline content and MDA content, respectively , One, three, three, four, distributed in 13 chromosomes. The five additive QTLs derived from wool were qSHDC-19-1, qSHDC-19-2, qSLNDC-5-1, qMDADC-24-1 and qMDADC-24-2, respectively, with additive effects of 0.10-0.22 , Explain the variation 9.4% ~ 25.8%. 【Conclusion】 These QTLs related to drought resistance contribute to marker-assisted selection of cotton drought-resistance markers.