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目的：探讨３′－ＨＶＲ探针及Ｓｏｕｔｈｅｒｎ杂交技术应用于实验小鼠遗传质量监测的可行性。方法：采用人源α－珠蛋白３′－ＨＶＲ探针，应用ＥＣＬ试剂盒，以ＨＲＰ标记进行Ｓｏｕｔｈｅｒｎ杂交，以化学发光法进行检测，对５种近交系小鼠（ＢＡＬＢ／ｃ、ＤＢＡ／２、Ｔ７３９、ＴＡ１、６１５）、１种封闭群小鼠（ＫＭ）及２种免疫缺陷小鼠（ＢＡＬＢ／ｃ－ｎｕ／ｎｕ及Ｃ．Ｂ－１７ＳＣＩＤ）进行ＤＮＡ指纹分析。结果：其不同近交小鼠的ＤＮＡ指纹图各异，而同种近交系的不同个体的ＤＮＡ指纹图相同，封闭群ＫＭ小鼠不同个体之间的ＤＮＡ指纹图存在一定差异。ＢＡＬＢ／ｃ－ｎｕ／ｎｕ小鼠、ＣＢ－１７ＳＣＩＤ小鼠不同个体之间的ＤＮＡ指纹图相同，并且与ＢＡＬＢ／ｃ小鼠的一致。结论：该探针及其Ｓｏｕｔｈｅｒｎ杂交技术可用于实验小鼠的遗传质量监测。
Objective: To investigate the feasibility of using 3’-HVR probe and Southern hybridization in genetic quality monitoring of experimental mice. Methods: The human hybridization was performed by using the human α-globin 3’-HVR probe, ECL kit and Southern blotting with HRP, and the chemiluminescence method was used to detect the expression of these genes in BALB / c, DBA / 2, T739, TA1,615), one closed group mouse (KM) and two immunodeficient mice (BALB / c-nu / nu and C.B- 17SCID). Results: DNA fingerprints of different inbred mice were different, while DNA fingerprinting of different individuals in the same kind of inbred strains was the same. DNA fingerprinting among different individuals in the closed-group KM mice was different. The DNA fingerprints between BALB / c-nu / nu mice and CB-17SCID mice were identical and consistent with BALB / c mice. Conclusion: The probe and its Southern hybridization can be used to monitor the genetic quality of mice.