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目的：研究槲皮素（Ｑｕｅ）对ＨＬ６０细胞中细胞溶质和胞膜蛋白激酶Ｃ（ＰＫＣ）、酪氨酸蛋白激酶（ＴＰＫ）活性的影响．方法：活细胞数的计数用苔盼兰拒染法；用组蛋白ⅢＳ、［γ３２Ｐ］ＡＴＰ与ＰＫＣ酶液一起保温测定ＰＫＣ活性；用聚谷氨酸·酪氨酸（４∶１）多肽）、［γ３２Ｐ］ＡＴＰ与ＴＰＫ酶液一起保温测定ＴＰＫ活性．结果：Ｑｕｅ对ＨＬ６０细胞的增殖有抑制作用，呈剂量依赖关系，处理４８小时后，其ＩＣ５０为２９（２２－３７）μｍｏｌ·Ｌ－１；在体外，Ｑｕｅ能强烈抑制细胞溶质ＰＫＣ和胞膜ＴＰＫ活性，其ＩＣ５０分别为：３１（２０－４８）μｍｏｌ·Ｌ－１，２４（１３－４５）μｍｏｌ·Ｌ－１，但不影响胞膜ＰＫＣ和细胞溶质ＴＰＫ活性．结论：这为Ｑｕｅ对癌细胞的生长有抑制作用与其抑制ＰＫＣ和／或ＴＰＫ有关提供了直接证据．
Objective: To study the effects of quercetin (Que) on cytosolic and membrane protein kinase C (PKC) and tyrosine protein kinase (TPK) activity in HL60 cells. Methods: The count of viable cells was determined by trypan blue exclusion assay. PKC activity was determined by incubating PKC enzyme with histone ⅢS and [γ32P] ATP. Polyglutamic acid and tyrosine (4:1) Polypeptide), [γ 32P] ATP and TPK enzyme solution was incubated with TPK activity. Results: Que inhibited the proliferation of HL-60 cells in a dose-dependent manner. After 48 hours of treatment, the IC50 was 29 (22-37) μmol·L-1. In vitro, Que inhibited the cytosolic PKC and The IC50 of membrane TPK activity was 31 (20-48) μmol·L-1,24 (13-45) μmol·L-1, but did not affect the membrane PKC and cytosolic TPK activity. Conclusion: This provides direct evidence that Que suppresses the growth of cancer cells in relation to its inhibition of PKC and / or TPK.