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目的 研究血管内皮生长因子C(VEGF C)对舌鳞癌癌周淋巴管的促增殖作用。方法 将VEGF C高表达的舌鳞癌细胞株(A组)接种于发育至第 6~8天的鸡胚绒毛尿囊膜 (CAM),设立空白质粒转染组(B组)和空白对照组 (C组), 5′ 核苷酸酶 (5′ Nase)染色观察瘤周淋巴管,淋巴管形态学测量统计分析淋巴管数密度(LVD)、截面面积以及周长的变化。结果 3组在CAM上均能成瘤,肿瘤组织对周围CAM的侵袭较弱,瘤体组织内未见淋巴管的生成;瘤周淋巴管形态学测量分析发现A组癌周淋巴管的LVD、截面面积和周长均高于B、C组,差异有统计学意义(P<0 01);B、C组之间差异无统计学意义(P>0 05)。结论 CAM是研究淋巴管的较理想的模型;VEGF C能够诱导癌周淋巴管扩张,这可能是VEGF C增加舌鳞癌颈淋巴转移的机制之一。
Objective To investigate the role of vascular endothelial growth factor C (VEGF C) in promoting the proliferation of lymphatic vessels around the tongue squamous cell carcinoma. Methods The tongue squamous cell carcinoma cell line with high expression of VEGF C (group A) was inoculated into the chick chorioallantoic membrane (CAM) of the chick embryo on the 6th to 8th day. The blank plasmid transfection group (group B) and blank control group (C group) and 5 ’Nase (5’ Nase) staining were used to observe the changes of lymphatic vessel number, cross section area and perimeter of the lymphatic vessels and lymphatic vessels. Results All of the three groups were capable of tumorigenesis on CAM. The invasion of tumor tissue to surrounding CAM was weak, and no lymphatic vessels were found in the tumor tissues. Peripheral lymphatic vessel morphology analysis revealed that LVD, Area and circumference were higher than those in group B and C (P <0.01). There was no significant difference between group B and C (P> 0.05). Conclusions CAM is an ideal model for studying lymphatic vessels. VEGF C can induce lymphangiogenesis in the peritumoral region, which may be one of the mechanisms of VEGF C increasing cervical lymph node metastasis of tongue squamous cell carcinoma.