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利用一种快速玉米基因组DNA提取方法,分别对Bt11、MON810、NK603、MIR604和TC1507这5种转基因玉米种子的胚和胚乳进行DNA提取。根据不同转基因玉米重组结构点,分别设计特异性引物,参考农业部转基因检测标准,通过对PCR体系的优化整合后发现,该玉米DNA快速提起法获得的PCR检测结果准确、可靠,在此基础之上建立一套多重PCR,使转基因检测的效率得到很大提高。
Using a rapid maize genomic DNA extraction method, the embryos and endosperm of five transgenic maize seeds, Bt11, MON810, NK603, MIR604 and TC1507, respectively, were extracted with DNA. According to different recombination structures of transgenic maize, specific primers were designed, reference to the Ministry of Agriculture transgenic testing standards, through the optimization and integration of the PCR system found that the rapid DNA amplification of corn DNA obtained PCR test results accurate and reliable, based on this On the establishment of a set of multiplex PCR, the efficiency of genetically modified testing has been greatly improved.