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目的:研究老鼠簕生物碱A(IA)及其衍生物乙酰老鼠簕生物碱A(ACO-IA)的抗炎镇痛作用及对小鼠的急性毒性、胃肠刺激作用。方法:采用改良寇氏法计算小鼠LD50并观察毒性作用,采用二甲苯致小鼠耳肿胀法、小鼠热板法、醋酸扭体法观察其抗炎镇痛作用,并观察连续灌胃给药对小鼠胃肠的影响。结果:IA的对小鼠的半数致死量即LD50值为2 198.87 mg.kg-1,ACO-IA对小鼠的LD50值为2 009.43 mg·kg-1。IA和ACO-IA灌胃给药对冰醋酸所引起的小鼠炎性疼痛有显著抑制作用,能够减轻二甲苯所致的小鼠耳肿胀程度,且作用与阿司匹林相当,而对小鼠胃刺激性比阿司匹林小。结论:按毒性分级标准2个化合物属于低毒性的药物,且均具有抗炎镇痛作用,能够减轻小鼠胃黏膜的损伤程度。
OBJECTIVE: To study the anti-inflammatory and analgesic effects of AChE-IA and its derivatives acetylcholine alkaloids A (ACO-IA) and its acute toxicity and gastrointestinal stimulation in mice. Methods: LD50 was calculated by modified Kovar method and the toxic effects were observed. Anti-inflammatory and analgesic effects were observed by xylene-induced mouse ear swelling, mouse hot plate method and acetic acid writhing. The effects of continuous intragastric administration Effect of medicine on mice gastrointestinal. Results: The half-lethal dose (LD50) of IA to mice was 2 198.87 mg.kg-1, and the LD50 of ACO-IA to mice was 2 009.43 mg · kg-1. Gavage administration of IA and ACO-IA significantly inhibited the inflammatory pain induced by glacial acetic acid in mice, which could reduce the degree of ear swelling in mice induced by xylene and had the same effect as aspirin, Sex is less than aspirin. Conclusion: The two compounds belong to low toxicity drugs according to the toxicity classification standard and have anti-inflammatory and analgesic effects, which can reduce the degree of gastric mucosal injury in mice.