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目的通过病例对照研究,探讨陕西省汉族人群血浆miRNA-491-5p水平和miRNA-491-5p(has-miR-491-5p)靶基因基质金属蛋白酶-9(MMP-9)单核苷酸多态性(SNP)改变与早发冠心病(pCAD)发生风险及预后的关系。方法连续收集pCAD病例270例,对照组300例。用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)检测has-miR-491-5p靶基因MMP-9rs1056628的多态性,同时比较各种基因型与pCAD发生的相关性。结果 rs1056628位点存在A-C多态性,与CC基因型(发生率42%)相比,携带CA、AA基因型的个体冠心病的发生率为31%,差异有统计学意义(P=0.045);携带CA/AA基因型冠心病在低水平总胆固醇(TC)、较低水平的低密度脂蛋白胆固醇(LDL-C)人群中的患病风险降低更为显著。结论 has-miRNA-491-5p靶基因MMP-9rs1056628C-A多态性改变可降低pCAD的发生率,携带C等位基因是pCAD发病的独立危险因素。
Objective To investigate the level of plasma miRNA-491-5p and the expression of matrix metalloproteinase-9 (MMP-9) of miRNA-491-5p (has-miR-491-5p) gene in Shaanxi Han population through case- Relationship between the change of status (SNP) and the risk and prognosis of premature coronary heart disease (pCAD). Methods Continuous collection of pCAD cases 270 cases, control group 300 cases. The polymorphism of MMP-9rs1056628 in has-miR-491-5p gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the correlation between various genotypes and pCAD was also compared. Results There was AC polymorphism at rs1056628. Compared with CC genotype (42% incidence), the prevalence of coronary heart disease was 31% in individuals with CA and AA genotypes, the difference was statistically significant (P = 0.045) ; The prevalence of coronary heart disease with CA / AA genotypes was significantly lower in the low-level total cholesterol (TC) and low-level low-density lipoprotein cholesterol (LDL-C) population. Conclusion The change of MMP-9rs1056628C-A polymorphism of has-miRNA-491-5p target gene can reduce the incidence of pCAD. Carrying C allele is an independent risk factor of pCAD.