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目的调查致泻性大肠杆菌(DEC)在福建省腹泻人群中的分布情况,为做好防治工作提供本底资料。方法对监测医院腹泻患者的粪便样本提取增菌液的DNA,再用实时荧光PCR(RT-PCR)技术进行相关病原菌检测,以了解5种病原菌在腹泻人群中的分布情况。结果检测206份样本,DEC总检出率为18.9%,各相关病原菌检出率:肠致病性大肠杆菌(EPEC)为8.3%,均为不典型肠致病性大肠杆菌;肠产毒性大肠杆菌(ETEC)为1.5%,均为产耐热肠毒素-SL阴性、不耐热型肠毒素-LT阳性;肠侵袭性大肠杆菌(EIEC)为1.9%,肠黏附性大肠杆菌(EAEC)为7.3%,肠出血性大肠杆菌(EHEC)未检出。结论 DEC相关病原菌以EPEC和EAEC为主,其次为EIEC和ETEC,未检出EHEC,与20世纪80年代相比,病原谱发生了变化。
Objective To investigate the distribution of diarrhea-causing Escherichia coli (DEC) in diarrhea in Fujian Province and provide background information for prevention and treatment. Methods The DNA of the enrichment broth was extracted from the stool samples of hospitalized patients with diarrhea. The pathogenic bacteria were detected by real-time fluorescence PCR (RT-PCR) to understand the distribution of the five pathogens in the diarrhea population. Results A total of 206 samples were detected. The total detection rate of DEC was 18.9%. The detection rate of all the relevant pathogens was 8.3% of intestinal pathogenic E.coli (EPEC), all of which were not typical enteropathogenic E.coli. Bacillus coli (ETEC) was 1.5%, all of which were heat-resistant enterotoxigenic-SL negative and heat-labile enterotoxin-LT positive; intestinal invasive Escherichia coli (EIEC) was 1.9%, intestinal adhering E. coli 7.3%, enterohemorrhagic Escherichia coli (EHEC) were not detected. Conclusion The pathogens of DEC-related pathogens are mainly EPEC and EAEC, followed by EIEC and ETEC, but no EHEC was detected. Compared with the 1980s, the pathogen spectrum changed.