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目的:观察糖基化终产物(advanced glycation end products,AGEs)对心肌微血管内皮细胞(cardiac microvascu-lar endothelial cells,CMECs)增殖能力、迁移能力,以及血管新生和管腔形成的作用和影响。方法:以不同浓度(100、200和400 mg/L)的AGEs作用于CMECs 48 h,分别采用MTT比色法检测细胞的增殖能力;用Transwell法检测细胞的迁移能力;用毛细血管管腔结构形成试验检测AGEs对血管新生的影响。结果:CMECs在以不同浓度(100、200及400 mg/L)的AGEs作用48 h后,MTT比色法检测显示,吸光值(分别为0.1195±0.0049、0.1422±0.0058及0.1783±0.0220)明显高于对照组(0.0955±0.0161,P<0.05,P<0.01);Transwell法检测细胞数[分别为(24.89±6.234)、(32.89±6.990)及(55.56±10.27)个]均高于对照组[(13.89±4.622)个,P<0.05,P<0.01];管样结构的长度[分别为(3.261±0.7016)、(4.737±1.129)及(6.687±1.308)mm/mm2]均高于对照组[(2.089±0.6723)mm/mm2,P<0.05,P<0.01]。结论:AGEs可以促进CMECs血管新生和管腔结构的形成,且与其浓度呈正相关。
OBJECTIVE: To observe the effects of advanced glycation end products (AGEs) on the proliferation, migration, angiogenesis and lumen formation of cardiac microvascu-lar endothelial cells (CMECs). Methods: CMECs were treated with different concentrations of AGEs (100, 200 and 400 mg / L) for 48 h. MTT assay was used to detect the proliferative ability of the cells. Transwell assay was used to detect the migration ability of the cells. Capillary lumen structure Formation of test to detect the effect of AGEs on angiogenesis. Results: MTT assay showed that the absorbance values of CMECs were significantly higher (0.1195 ± 0.0049, 0.1422 ± 0.0058 and 0.1783 ± 0.0220, respectively) after treated with different concentrations of AGEs (100, 200 and 400 mg / L) In the control group (0.0955 ± 0.0161, P <0.05, P <0.01), the number of cells detected by Transwell method [(24.89 ± 6.234), (32.89 ± 6.990) and (55.56 ± 10.27) (13.89 ± 4.622), P <0.05, P <0.01]. The lengths of tube-like structures [(3.261 ± 0.7016), (4.737 ± 1.129) and (6.687 ± 1.308) mm / mm2] [(2.089 ± 0.6723) mm / mm2, P <0.05, P <0.01]. CONCLUSION: AGEs can promote the formation of angiogenesis and lumen structure in CMECs, and positively correlated with its concentration.