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目的:建立UPLC同时测定彝医药食同源植物多星韭不同部位腺嘌呤、鸟嘌呤核苷、胸腺嘧啶核苷、腺嘌呤核苷的方法,比较不同采收期不同部位4种核苷类成分的含量。方法:采用UPLC法,色谱柱为ACQUITY UPLC HSS T3(100 mm×2.1mm,1.8μm),流动相为甲醇-水,梯度洗脱,柱温30℃,体积流量0.2 m L/min,进样量2μL,检测波长为260 nm。结果:腺嘌呤、鸟嘌呤核苷、胸腺嘧啶核苷、腺嘌呤核苷含量分别在6.80~95.20μg/m L、6.70~93.80μg/m L、6.50~91.00μg/m L、7.20~100.80μg/m L线性范围内与峰面积呈良好线性关系,r为0.9995~0.9999,平均回收率为98.25%~101.38%,RSD≤2.32%;4种核苷类成分总含量在花期前>花期后,花蕾>叶>种子>根。结论:建立的方法操作简单、稳定性好、准确可靠,可用于同时测定药食同源植物多星韭中4种核苷类成分的含量,为开发利用多星韭提供依据。
OBJECTIVE: To establish a method for the simultaneous determination of adenine, guanosine, thymidine and adenosine in different parts of Yi-xi, a homoeologous plant of Yi medicine by UPLC and to compare four nucleoside components in different parts of different harvest time Content. Methods: The UPLC method was used. The column was ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 μm). The mobile phase consisted of methanol-water with a gradient elution. The column temperature was 30 ℃ and the volume flow rate was 0.2 mL / Amount of 2μL, detection wavelength of 260 nm. Results: The contents of adenine, guanosine, thymidine and adenosine were 6.80 ~ 95.20μg / m L, 6.70 ~ 93.80μg / m L, 6.50 ~ 91.00μg / m L and 7.20 ~ 100.80μg / m L linear range with the peak area showed a good linear relationship, r was 0.9995 ~ 0.9999, the average recovery was 98.25% ~ 101.38%, RSD ≤ 2.32%; 4 kinds of nucleosides content in the pre-flowering> Buds> Leaves> Seeds> Roots. Conclusion: The established method is simple, stable, accurate and reliable, and can be used for the simultaneous determination of four nucleoside components in the medicinal and homologous plants Polygonum Allii, providing the basis for the development and utilization of Polygonum Allium.