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为研究低密度脂蛋白经细胞修饰后对细胞受体的影响及前列腺素E2的作用，利用免疫细胞化学和生物亲和素-酶联免疫吸附法分别检测小鼠腹腔巨噬细胞清道夫受体、小鼠皮肤纤维母细胞低密度脂蛋白受体的结合活性，结果发现修饰组的巨噬细胞胞浆及胞膜有强阳性黄褐色颗粒，而药物组仅中度着色；修饰组低密度脂蛋白与其受体结合量（54±13μg／g细胞蛋白）较对照组（144±μg／g细胞蛋白）显著下降（P＜0．01）；药物组受体结合量（100±11μg／g细胞蛋白）较修饰组明显提高（P＜0．05）。表明细胞修饰低密度脂蛋白能够刺激清道夫受体活性，抑制低密度脂蛋白受体活性；前列腺素E2（20mg／L）可对抗细胞修饰低密度脂蛋白对此二种受体的作用。
In order to study the effect of low density lipoprotein (LDL) on cell receptors after cell modification and the effect of prostaglandin E2, immunocytochemistry and biotin-enzyme-linked immunosorbent assay were used to detect the expression of scavenger receptor , Mouse skin fibroblasts low density lipoprotein receptor binding activity and found that the modified group of macrophages macrophages and plasma membrane strong positive brown particles, and the drug group only moderate staining; modified group of low-density lipids (54 ± 13μg / g cell protein) was significantly lower than that of the control group (144 ± μg / g cell protein) (P <0.01). The amount of receptor binding in the drug group (100 ± 11μg / g cell Protein) than the modified group was significantly increased (P <0.05). The results showed that cell-modified LDL could stimulate scavenger receptor activity and inhibit LDL receptor activity. Prostaglandin E2 (20 mg / L) could antagonize the effect of cell-modified low density lipoprotein on these two receptors.