【摘 要】
：
Rheumatoid factors(RFs) are the characteristic autoantibodies of rheumatoid arthritis. Recent researches in our laboratory showed that the immobilized single-st
【机 构】
：
Key Laboratory of Bioactive Materials
【基金项目】
：
国家重点基础研究发展计划(973计划);Tianjin-Nankai University Co-Construction Foundation and China-UK Collaboration Project
论文部分内容阅读
Rheumatoid factors(RFs) are the characteristic autoantibodies of rheumatoid arthritis. Recent researches in our laboratory showed that the immobilized single-stranded DNA(ss-DNA) immunoadsorbent can selectively remove RFs from the serum of patients. In the present paper are studied the modification of argininine, tryptophan, lysine residues and carboxyl terminus of IgGRF, which was separated from patients′ serum, with 1,2-cyclohexanedione(CHD), N-bromosuccinimide(NBS), pyridoxal 5′-phosphate(PP) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC) respectively, and their effects on the adsorption capacity of the immobilized ss-DNA immunoadsorbent for IgGRF. After the specific modification, the corresponding adsorption capacities of the adsorbents were changed from 48%, 46%, 44% and 54% to 84%, 14%, 21% and 81%, respectively. These results indicate that the electrostatic or ionic-bonding is essential for the interaction between ss-DNA and IgGRF.
其他文献
A practical and scalable synthesis route of acetylphenylboronic acids is described. Bromoacetophenones(compounds 3a, 3b) were ketalized with ethylene glycol and
A technique for timescale analysis of spectral lags performed directly in the time domain is developed. Simulation studies are made to compare the time domain t
利用水热合成法合成了分子组成为(C6H11NH3)5H(P2Mo5O23)4H2O的杂多化合物, 用单晶X-ray衍射方法测定了它的结构,该晶体属于单斜晶系,空间群P21/c, a = 12.830(3), b = 14.84
Using the archived optical spectra of NGC 5548 between 1989 and 2001,we derived the optical spectral index by fitting the spectra in wavelength windows unaffect
The pion and tensor vacuum susceptibilities are calculated in the framework of the renormalizable DysonSchwinger equations. A comparison with the results of oth
The intermediate of d-biotin, (3aS,6aR)-1,3-dibenzyltetrahydro-1H-thieno[3,4-d]-imidazole-2(3H)-one-4-ylidenepentanoil acid was synthesized from (3aS,6aR)-1,3-d
The encapsulation of stearic acid coated nanometer CaCO3 by a polystyrene (PS) network via emulsion polymerization is described, where γ-methacryloxypropyltrim
The influences of silica volume fraction, electrolyte concentration and pH value upon the stress dependence of elastic modulus G′and viscous modulus G″ were i
The interactions between four high bioactive diperoxovanadate complexesKn[OV(O2)2L]·mH2O (n = 1 ～3, m = 1～5, L = oxalate (abbr. oxa), picolinate (abbr. pic), bip