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目的:研究缬沙坦对大鼠心肌梗死(MI)后电压门控钾通道(Kv)9.X(Kv9.1、Kv9.2、Kv9.3)变化的影响。方法:通过结扎大鼠冠状动脉左前降支近端建立MI大鼠模型,手术后存活大鼠随机分为MI组(7、30d)和缬沙坦组(7、30d:缬沙坦30mg/kg,qd),同时设立相应假手术组(7、30d)。MI组和假手术组对应给予等量0.85%氯化钠溶液。应用半定量RT-PCR方法检测非梗死左室心肌Kv9.1、Kv9.2、Kv9.3mRNA量。结果:缬沙坦组与MI组比较,无论7d或是30d,Kv9.1、Kv9.2、Kv9.3mRNA量均明显升高(P<0.05或P<0.01);与假手术组比较,无论7d或是30d,Kv9.1、Kv9.2、Kv9.3mRNA量差异均无统计学意义;MI组与假手术组比较,无论7d或是30d,Kv9.1、Kv9.2、Kv9.3mRNA量均明显降低(P<0.05或P<0.01);MI30d时与7d时比较Kv9.1、Kv9.2、Kv9.3mRNA量下降(均P<0.05)。结论:缬沙坦可以显著逆转MI后Kv9.1、Kv9.2、Kv9.3mRNA表达的下调,且该作用迅速而完全。
Objective: To investigate the effects of valsartan on the changes of voltage-gated potassium channel (Kv) 9.X (Kv9.1, Kv9.2, Kv9.3) after myocardial infarction in rats. Methods: MI rats were established by ligation of the proximal left anterior descending coronary artery in rats. After the operation, the rats were randomly divided into MI group (7, 30d) and valsartan group (7, 30d: valsartan 30mg / kg , qd), while setting up the corresponding sham operation group (7,30 d). MI group and sham group corresponding to the same amount of 0.85% sodium chloride solution. The amount of Kv9.1, Kv9.2 and Kv9.3 mRNA in non-infarcted left ventricular myocardium was detected by semi-quantitative RT-PCR. Results: Compared with the MI group, the levels of Kv9.1, Kv9.2 and Kv9.3 mRNA in the valsartan group were significantly increased (P <0.05 or P <0.01) at 7 and 30 days, respectively. Compared with the sham-operated group, There was no significant difference in Kv9.1, Kv9.2 and Kv9.3 mRNA levels between the MI group and the sham operation group at 7d or 30d (P <0.05 or P <0.01). The levels of Kv9.1, Kv9.2 and Kv9.3 mRNA decreased on the 30th and the 30th day after MI (all P <0.05). CONCLUSION: Valsartan can significantly reverse the down-regulation of Kv9.1, Kv9.2 and Kv9.3 mRNA expression after MI, and the effect is rapid and complete.