论文部分内容阅读
目的:评价Luminex NxTAGn TM RPP对17种呼吸道病原体的检测性能。n 方法:通过比较Luminex NxTAGn TM RPP试剂盒与一代测序检测临床标本的结果，以评价Luminex NxTAGn TM RPP试剂盒的检测性能。n 结果:与一代测序相比，Luminex NxTAGn TM RPP试剂盒对流感病毒A型（FluA）、2009甲型H1N1流感病毒（H1N1pdm09）、H3N2亚型流感病毒（H3N2）、流感病毒B型（FluB）、副流感病毒2型（PIV 2）、副流感病毒3型（PIV 3）、副流感病毒4型（PIV 4）、肺炎衣原体（CP）和冠状病毒HKU1（CoV HKU1）检测具有高度一致性；对腺病毒（ADV）、冠状病毒229E（CoV 229E）、冠状病毒OC43（CoV OC43）、呼吸道合胞病毒（RSV）、鼻病毒（RV）、博卡病毒（BoV）的检测结果具有中度一致性。然而对于副流感病毒1型（PIV 1）、偏肺病毒（MPV）两种方法检测一致性较差。n 结论:Luminex NxTAGn TM RPP试剂盒本身具有检测准确、高通量与操作时间短等特点，对大多数呼吸道病原体的检测表现出良好的性能。n “,”Objective:To evaluate the detection performance of Luminex NxTAGn TM RPP on 17 respiratory pathogens.n Methods:The detection performance of the Luminex NxTAGn TM RPP kit was evaluated by comparing the detection results of clinical specimens with those by the sanger sequencing.n Results:The results of Luminex NxTAGn TM RPP kit for influenza virus type A (FluA), influenza A virus (H1N1) pdm09, influenza A virus subtype H3N2 (H3N2), influenza B virus (FluB), parainfluenza virus type 2 (PIV 2), parainfluenza type 3 (PIV 3), parainfluenza type 4 (PIV 4), Chlamydia pneumonia (CP) and coronavirus HKU1 (CoV HKU1) were highly consistent to the results by the Sanger sequencing. The results of adenovirus (ADV), coronavirus 229E (CoV 229E), coronavirus OC43 (CoV OC43), respiratory syncytial virus (RSV), rhinovirus (RV) and Bokavirus (BoV) were moderately consistent to the results of the Sanger sequencing while the consistency of reuslts for parainfluenza virus type 1 (PIV 1) and metapneumovirus (MPV) by the methods was poor.n Conclusions:Luminex NxTAGn TM RPP kit was characterized by accurate detection, high throughput and short operation time with good performance in the detection of most respiratory pathogens.n